Determination of deracoxib in feline plasma samples using high performance liquid chromatography

被引：11
作者：
Cox, SK
Roark, J
Gassel, A
Tobias, K
机构：
[1] Univ Tennessee, Coll Vet Med, Dept Comparat Med, Knoxville, TN 37966 USA
[2] Univ Tennessee, Coll Vet Med, Dept Small Anim Clin Sci, Knoxville, TN 37966 USA
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 819卷 / 01期
关键词：
COX-2; inhibitors; deracoxib; HPLC;
D O I：
10.1016/j.jchromb.2005.01.025
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new HPLC procedure for the determination of deracoxib, a selective cyclooxygenase-2 inhibitor, has been developed and validated. Following a liquid-liquid extraction using isopropyl alcohol and chloroform, samples were separated by isocratic reversed-phase HPLC on an Atlantis C-18 column and quantified using UV detection at 252 nm. The mobile phase was a mixture of 10 mM potassium phosphate (pH 4.5) and acetonitrile, with a flow-rate of 1.0 ml/min. The procedure produced a linear curve over the concentration range 10-1500 ng/ml. The development of the assay allowed the determination of pharmacokinetic parameters after oral administration of deracoxib in cats and would be suitable for other pharmacokinetic studies. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：181 / 184
页数：4
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