Mass Spectrometry Imaging, Laser Capture Microdissection, and LC-MS/MS of the Same Tissue Section

被引:61
作者
Dilillo, Marialaura [1 ,2 ]
Pellegrini, Davide [1 ,3 ]
Ait-Belkacem, Rima [1 ]
de Graaf, Erik L. [1 ]
Caleo, Matteo [4 ]
McDonnell, Liam A. [1 ,5 ]
机构
[1] Fdn Pisana Sci ONLUS, I-56121 Pisa, Italy
[2] Univ Pisa, Dept Chem & Ind Chem, I-56126 Pisa, Italy
[3] Scuola Normale Super Pisa, NEST, I-56127 Pisa, Italy
[4] CNR, Neurosci Inst, I-56124 Pisa, Italy
[5] Leiden Univ, Med Ctr, Ctr Prote & Metabol, NL-2333 ZA Leiden, Netherlands
关键词
mass spectrometry imaging; microprotcomics; laser capture microdissection; molecular histology; localized microproteomics; multimodal analysis; EXTRACTION SURFACE-ANALYSIS; LMD-ICP-MS; PARAFFIN-EMBEDDED TISSUE; PROTEIN DIGESTION; DRUG DISTRIBUTION; BRAIN-TISSUE; MALDI-TOF; RESOLUTION; HETEROGENEITY; METABOLITES;
D O I
10.1021/acs.jproteome.7b00284
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mass spectrometry imaging (MSI) is able to simultaneously record the distributions of hundreds of molecules directly from tissue. Rapid direct tissue analysis is essential for MSI in order to maintain spatial localization and acceptable measurement times. The absence of an explicit analyte separation/purification step means NISI lacks the depth of coverage of LC-MS/MS. In this work, we demonstrate how atmospheric pressure MALDI-MSI enables the same tissue section to be first analyzed by MSI, to identify regions of interest that exhibit distinct molecular signatures, followed by localized proteomics analysis using laser capture microdissection isolation and LC-MS/MS.
引用
收藏
页码:2993 / 3001
页数:9
相关论文
共 45 条
[1]   Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria [J].
Aichler, Michaela ;
Elsner, Mareike ;
Ludyga, Natalie ;
Feuchtinger, Annette ;
Zangen, Verena ;
Maier, Stefan K. ;
Balluff, Benjamin ;
Schoene, Cedrik ;
Hierber, Ludwig ;
Braselmann, Herbert ;
Meding, Stephan ;
Rauser, Sandra ;
Zischka, Hans ;
Aubele, Michaela ;
Schmitt, Manfred ;
Feith, Marcus ;
Hauck, Stefanie M. ;
Ueffing, Marius ;
Langer, Rupert ;
Kuster, Bernhard ;
Zitzelsberger, Horst ;
Hoefler, Heinz ;
Walch, Axel K. .
JOURNAL OF PATHOLOGY, 2013, 230 (04) :410-419
[2]   Mass spectrometry imaging is moving toward drug protein co-localization [J].
Ait-Belkacem, Rima ;
Sellami, Lyna ;
Villard, Claude ;
DePauw, Edwin ;
Calligaris, David ;
Lafitte, Daniel .
TRENDS IN BIOTECHNOLOGY, 2012, 30 (09) :466-474
[3]   De novo discovery of phenotypic intratumour heterogeneity using imaging mass spectrometry [J].
Balluff, Benjamin ;
Frese, Christian K. ;
Maier, Stefan K. ;
Schoene, Cedrik ;
Kuster, Bernhard ;
Schmitt, Manfred ;
Aubele, Michaela ;
Hoefler, Heinz ;
Deelder, Andre M. ;
Heck, Albert J. R. ;
Hogendoorn, Pancras C. W. ;
Morreau, Johannes ;
Altelaar, A. F. Maarten ;
Walch, Axel ;
McDonnell, Liam A. .
JOURNAL OF PATHOLOGY, 2015, 235 (01) :3-13
[4]   Classification of HER2/neu Status in Gastric Cancer Using a Breast-Cancer Derived Proteome Classifier [J].
Balluff, Benjamin ;
Elsner, Mareike ;
Kowarsch, Andreas ;
Rauser, Sandra ;
Meding, Stephan ;
Schuhmacher, Christoph ;
Feith, Marcus ;
Herrmann, Ken ;
Rocken, Christoph ;
Schmid, Roland M. ;
Hofler, Heinz ;
Walch, Axel ;
Ebert, Matthias P. .
JOURNAL OF PROTEOME RESEARCH, 2010, 9 (12) :6317-6322
[5]   Scaling down the bioimaging of metals by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) [J].
Becker, J. S. ;
Niehren, S. ;
Matusch, A. ;
Wu, B. ;
Hsieh, H. -F. ;
Kumtabtim, U. ;
Hamester, M. ;
Plaschke-Schluetter, A. ;
Salber, D. .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2010, 294 (01) :1-6
[6]   High-resolution MALDI-FT-ICR MS imaging for the analysis of metabolites from formalin-fixed, paraffin-embedded clinical tissue samples [J].
Buck, Achim ;
Ly, Alice ;
Balluff, Benjamin ;
Sun, Na ;
Gorzolka, Karin ;
Feuchtinger, Annette ;
Janssen, Klaus-Peter ;
Kuppen, Peter J. K. ;
van de Velde, Cornelis J. H. ;
Weirich, Gregor ;
Erlmeier, Franziska ;
Langer, Rupert ;
Aubele, Michaela ;
Zitzelsberger, Horst ;
Aichler, Michaela ;
Walch, Axel .
JOURNAL OF PATHOLOGY, 2015, 237 (01) :123-132
[7]   Online, Absolute Quantitation of Propranolol from Spatially Distinct 20-and 40-μm Dissections of Brain, Liver, and Kidney Thin Tissue Sections by Laser Microdissection-Liquid Vortex Capture-Mass Spectrometry [J].
Cahill, John F. ;
Kertesz, Vilmos ;
Weiskittel, Taylor M. ;
Vavrek, Marissa ;
Freddo, Carol ;
Van Berkel, Gary J. .
ANALYTICAL CHEMISTRY, 2016, 88 (11) :6026-6034
[8]   Laser dissection sampling modes for direct mass spectral analysis [J].
Cahill, John F. ;
Kertesz, Vilmos ;
Van Berkel, Gary J. .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2016, 30 (05) :611-619
[9]   Characterization and Application of a Hybrid Optical Microscopy/Laser Ablation Liquid Vortex Capture/Electrospray Ionization System for Mass Spectrometry Imaging with Sub-micrometer Spatial Resolution [J].
Cahill, John F. ;
Kertesz, Vilmos ;
Van Berkel, Gary J. .
ANALYTICAL CHEMISTRY, 2015, 87 (21) :11113-11121
[10]   Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry [J].
Casadonte, Rita ;
Caprioli, Richard M. .
NATURE PROTOCOLS, 2011, 6 (11) :1695-1709