Deep-red to near-infrared fluorescent dyes: Synthesis, photophysical properties, and application in cell imaging

被引：14

作者：

Li, Qi ^{[1
,2
]}

Liu, Weimin ^{[1
]}

Wu, Jiasheng ^{[1
]}

Zhou, Bingjiang ^{[1
]}

Niu, Guangle ^{[1
,2
]}

Zhang, Hongyan ^{[1
]}

Ge, Jiechao ^{[1
]}

Wang, Pengfei ^{[1
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 164卷

基金：

中国国家自然科学基金;

关键词：

Near-infrared fluorescent dyes; Water solubility; Lysosomes; Cell imaging; IN-VIVO; LIVING CELLS; PROBE; DERIVATIVES; CONJUGATE; COUMARIN; VITRO; TRACKING; CYSTEINE; SHIFT;

D O I：

10.1016/j.saa.2016.03.042

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

More and more attention has been paid to the design of new fluorescent imaging agents with good photostability and water solubility, especially those with emissions in the deep-red and near-infrared regions. In this work, we designed and synthesized four novel fluorescent dyes with deep-red or NIR fluorescence by hybridizing coumarin and pyronin moieties based on our previous work. Introduction of carboxylic acid in the dyes not only imparted the dyes with water solubility but also provided a versatile sensing platform for designing the fluorescent probes and sensors of biomolecules. The photophysical properties of these new dyes were investigated through absorption and fluorescence spectroscopy. Cell imaging experiments showed that esterification products could selectively stain lysosomes with good photostability, thereby indicating that they could be useful in the development of fluorescent probes for bioimaging. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：8 / 14

页数：7

共 42 条

[1] Dyes based on 5,10-dihydrophenophosphazine. Part I: Disazo direct dyes [J].