Analysis of paralytic shellfish toxins using high-field asymmetric waveform ion mobility spectrometry with liquid chromatography-mass spectrometry

被引:25
作者
Beach, Daniel G. [1 ]
Melanson, Jeremy E. [2 ]
Purves, Randy W. [3 ]
机构
[1] Natl Res Council Canada, Measurement Sci & Stand, Halifax, NS B3H 3Z1, Canada
[2] Natl Res Council Canada, Measurement Sci & Stand, Ottawa, ON K1A 0R6, Canada
[3] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 5A8, Canada
关键词
Differential mobility spectrometry (DMS); Paralytic shellfish poisoning (PSP); Algal toxins; Marine biotoxins; Liquid chromatography-mass spectrometry; High-field asymmetric waveform ion mobility spectrometry (FAIMS); QUANTITATIVE-DETERMINATION; LABORATORY VALIDATION; FAIMS; SENSITIVITY; SEPARATION; OYSTERS; MUSSELS;
D O I
10.1007/s00216-015-8488-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The analysis of paralytic shellfish toxins (PSTs) by liquid chromatography-mass spectrometry remains a challenge because of their high polarity, large number of analogues and the complex matrix in which they occur. Here we investigate the potential utility of high-field asymmetric waveform ion mobility spectrometry (FAIMS) as a gas-phase ion separation tool for analysis of PSTs by mass spectrometry. We investigate the separation of PSTs using FAIMS with two divergent goals: using FAIMS as a primary separation tool for rapid screening by electrospray ionization (ESI)-FAIMS-MS or combined with LC in a multidimensional LC-ESI-FAIMS-MS separation. First, a survey of the parameters that affect the sensitivity and selectivity of PST analysis by FAIMS was carried out using ESI-FAIMS-MS. In particular, the use of acetonitrile as a gas additive in the carrier gas flow offered good separation of all PST epimeric pairs. A second set of FAIMS conditions was also identified, which focussed PSTs to a relatively narrow CV range allowing development of an LC-ESI-FAIMS-MS method for analysis of PST toxins in complex mussel tissue extracts. The quantitative capabilities of this method were evaluated by analysing a PST containing mussel tissue matrix material. Results compared favourably with analysis by an established LC-post-column oxidation-fluorescence method with recoveries ranging from 70 to 106 %, although sensitivity was somewhat reduced. The current work represents the first successful separation of PST isomers using ion mobility and shows the promise of FAIMS as a tool for analysis of algal biotoxins in complex samples and outlines some critical requirements for its future improvement.
引用
收藏
页码:2473 / 2484
页数:12
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