H727 Multicellular Spheroids and Its Resistance to Antitumor Drugs Sunitinib and Axitinib

被引：4

作者：

Wang, Zhengyang ^{[1
]}

Chen, Hao ^{[2
,3
]}

Zhou, Naizhen ^{[1
]}

Zhang, Tianzhu ^{[1
]}

Ren, Pengfei ^{[1
]}

Chen, Gang ^{[2
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, Natl Demonstrat Ctr Expt Biomed Engn Educ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

[2] Nanjing Univ, Sch Med, Affiliated Hosp, Dept Gen Surg,Nanjing Drum Tower Hosp, Nanjing 210008, Peoples R China

[3] Southeast Univ, Sch Med, Nanjing 210009, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2018年 / 18卷 / 12期

关键词：

Neuroendocrine Tumor; Three-Dimensional Culture; Sunitinib; Axitinib; VEGFR Inhibitor; PANCREATIC NEUROENDOCRINE TUMORS; CANCER-THERAPY; CELLS; DELIVERY; GENE; EXPRESSION; CARCINOMA; PROGRESS; NANOPARTICLES; CHITOSAN;

D O I：

10.1166/jnn.2018.16030

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The three-dimensional (3D) culture model of neuroendocrine tumor H727 cells was established by using the agarose gel as culture matrix, which provided a new method for drug screening of neuroendocrine tumors. As VEGFR inhibitor, sunitinib and axitinib were applied to inhibit human neuroendocrine H727 cell line in two-dimensional (2D) and 3D culture models. The inhibitory rate of H727 cells with different drug concentration were assessed by CCK-8 assay method and combined with using the FDA/PI double staining and the digital microscope analysis system. When the concentration of sunitinib >= 4.0 mu mol/L, the H727 spheroids began to split, and the apoptosis of H727 cells occurred, the sizes of multicellular spheroids was significantly reduced in the groups of high-dose axitinib. These results illustrated that sunitinib and axitinib can effectively inhibit the growth and proliferation of neuroendocrine tumor H727 cells. Sunitinib and axitinib can also promote apoptosis of H727 cells.

引用

页码：8078 / 8084

页数：7