Plant-pathogen interaction between Corynespora cassiicola and Carica papaya

Prior research has indicated that Corynespora cassiicola was pathogenic to C papaya under optimum greenhouse conditions. The aims of this study were to examine the physical aspects of infection by C. cassiicola on the leaf surfaces of the resistant C. papaya. Corynespora cassiicola was isolated from infected Carica papaya, and maintained in the at 30 degrees C on V8 (half-strength) agar. Conidia were collected from 14 day old V8 (half-strength) agar plates by washing the surface with sterile distilled water and using a rubber spatula to rub for further experiment. At the end of the experiment, suggest ways to manage the infection. Observation of the infection was done by light microscopy which involved cross-section and leaf clearing method and also by scanning electron microscopy (SEM). Formation of many appressorium and 59.5% direct infection through direct infection (cuticle) instead of trough stomata were proved the C. cassiicola is great potential pathogen. The character of C. cassiicola's conidia germination was identified from both end and randomly from any septate. In Cross-section on both light and Scanning Electron Microscope shows the present of C. cassiicola part in the plant cell as proved that the infection was happened. The symptom of leaf spot disease was closed to leaf vein also proven by the compact of C. cassiicola mycelium view under SEM. Hence, as the pathogen seem to be major for the C. papaya, early detection was crucial for these diseases in order to control the dissemination.