Human cytomegalovirus (CMV)-specific CD8+ T cell responses are reduced in HIV-infected individuals with a history of CMV disease despite CD4+ T cell recovery

Cytomegalovirus (CMV)-specific immunity was investigated in human immunodeficiency virus (HIV)-infected individuals. A case-controt (1:2) study was performed with cases defined as having a history of CMV end-organ disease (n=15) and controls (n=30) matched by current CD4(+) T cell count. CMV-specific CD8(+) T cells responses were quantified using the high throughput Quantiferon((R))-CMV test (Cellestis, Melbourne, Australia). 40/44 (91%) had a positive Quantiferone((R))-CMV test and the magnitude of response to CMV peptides correlated significantly with response to mitogen (p < 0.0001) but not with CD4(+) T cell count at the time of testing, CD4+ T cell nadir or HIV viral load. Cases had a significantly tower Quantiferone((R))-CMV test than controls but there was no significant difference in response to mitogen or other antigens. In individuals with a history of CMV disease, CMV-specific CD8(+) T cell responses are reduced even in the setting of CD4(+) Tcell reconstitution. (c) 2007 Elsevier Inc. All rights reserved.