High performance liquid chromatographic determination of phenolic compounds in seed exudates of Festuca arundinacea and F-pratense

Derivatives of benzoic and cinnamic (caffeic, protocatechuic, vanillic, ferulic, p-coumaric, p-hydroxybenzoic and syringic) acids and 3,4-dihydroxybenzaldehyde in seed exudates of Festuca arundinacea cv, "Kora" and F. pratense cv. "Otava" have been separated and quantified on a Zorbax SE C-18 Rapid Resolution(TM) column with linear gradient elution using a mobile phase containing acetonitrile and 0.5 % acetic acid. The USP tailing factors were less than 1.15 for all peaks. Strictly linear calibration curves (r > 0.9997) with limits of detection of 90-880 nM were obtained for all analytes in the concentration ranges from units or tenths up to 600 muM. RP 105 and OASIS(TM) HLB polymeric sorbents have been shown to be the best of the tested sorbents for the separation and preconcentration of phenolic compounds from both plant materials. The solid-phase extraction procedures have been compared with classical liquid-liquid extractions and consistent results were obtained, Copyright (C) 2000 John Wiley & Sons, Ltd.