Identification of human basic fetoprotein as glucose-6-phosphate isomerase by using N- and C-terminal sequence tags and terminal tag database

被引：1

作者：

Kuyama, Hiroki ^{[1
]}

Yoshizawa, Akiyasu C. ^{[1
]}

Nakajima, Chihiro ^{[1
]}

Hosako, Mutsumi ^{[1
]}

Tanaka, Koichi ^{[1
]}

机构：

[1] Shimadzu Co Ltd, Koichi Tanaka Lab Adv Sci & Technol, Nakagyo Ku, Kyoto 6048511, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2015年 / 112卷

基金：

日本学术振兴会;

关键词：

Basic fetoprotein; N- and C-terminal sequencing; MALDI mass spectrometry; Terminal tag database; protein identification; DESORPTION/IONIZATION MASS-SPECTROMETRY; PROTEIN IDENTIFICATION; ARGININE RESIDUES; CARBOXYLIC-ACIDS; PEPTIDES; DERIVATIZATION; FRAGMENTATION; DERIVATIVES; CONVERSION; DIGESTS;

D O I：

10.1016/j.jpba.2015.04.032

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Human basic fetoprotein (BFP), found in fetal serum and tissue extracts as well as in extracts of various cancer tissues, has long been known as a marker protein for cancers; however, the primary sequence has not yet been reported. This paper describes the identification of BFP using the N- and C-terminal amino acid sequence tags (Ac-AALTRDPQFQ and QQREARVQ respectively) clarified by mass spectrometry-based methods, and a terminal tag database (ProteinCarta). In this study, BFP was identified as glucose-6-phosphate isomerase (G6PI_HUMAN). (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：116 / 125

页数：10

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