RFLP mapping of loci controlling self-incompatibility in Brassica campestris and their comparative mapping with B. napus and B. oleracea

被引：3

作者：

Panigrahi, J. ^{[1
]}

Kole, P. ^{[2
]}

Kole, C. ^{[2
]}

机构：

[1] Majhighariani Inst Technol & Sci, Div Biotechnol, Rayagada 765017, Orissa, India

[2] Deemed Univ, Allahabad Agr Inst, Allahabad 211007, Uttar Pradesh, India

来源：

BIOLOGIA PLANTARUM | 2011年 / 55卷 / 01期

关键词：

linkage analysis; molecular map; RI population; SLG gene; S-LOCUS; ARABIDOPSIS-THALIANA; LINKAGE MAP; RAPA; GENES; CDNA; GLYCOPROTEIN; POLYMORPHISM; POPULATIONS; EXPRESSION;

D O I：

10.1007/s10535-011-0007-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

RFLP analysis of a cDNA probe SLG6, governing self incompatibility (SI) in Brassica oleracea, using a recombinant inbred population of Brassica campestris followed by genetic linkage analysis led to the detection of two marker loci, SLG6a and SLG6b controlling SI. SLG6a was mapped in linkage group (LG) 9 and was flanked by the RFLP markers ec4f10 (6.4 cM) and wg5b9 (4.2 cM). SLG6b positioned in LG 2 and was flanked by the RFLP markers wg2d11 (9.9 cM) and ec4e7 (26.9 cM). These results indicated the scope of marker-aided introgression of these genes into self-compatible genotypes for production of SI lines suitable for hybridization in B. campestris. Comparative mapping of LG 9 containing SLG6b with corresponding linkage groups of B. oleracea (BO 2) and B. napus (BN 16) led to the detection of small homologous regions with SLG6 locus linked with another RFLP locus. This evidenced for homology of the SLG genes across Brassica species and possibility of using any single cloned SLG gene for development of SI lines in any Brassica species.

引用

页码：54 / 60

页数：7

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