BCL6 Expression Correlates With the t(1;19) Translocation in B-Lymphoblastic Leukemia

被引：15

作者：

Deucher, Anne M. ^{[1
]}

Qi, Zhongxia ^{[1
]}

Yu, Jingwei ^{[1
]}

George, Tracy I. ^{[2
]}

Etzell, Joan E. ^{[1
]}

机构：

[1] Univ Calif San Francisco, San Francisco, CA 94143 USA

[2] Univ New Mexico, Albuquerque, NM 87131 USA

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2015年 / 143卷 / 04期

关键词：

BCL6; t(1; 19); B-ALL; B-lymphoblastic leukemia; TCF3(E2A)-PBX1; GENE-EXPRESSION; CHROMOSOMAL TRANSLOCATION; SOMATIC HYPERMUTATION; CELL; CHILDHOOD; LYMPHOMA; PROTEINS; ADULT; E2A; DIFFERENTIATION;

D O I：

10.1309/AJCPO4U4VYAAOTEL

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Objectives: Study to date suggests that BCL6 protein expression in B-cell neoplasia predominates in germinal center derived tumors, but less is known regarding its expression in B-lymphoblastic leukemia. Therefore, we designed a comprehensive study of BCL6 expression in B-lymphoblastic leukemia. Methods: BCL6, LMO, and HGAL protein expression in B-lymphoblastic leukemia was investigated using immunohistochemical staining of paraffin-embedded bone marrow specimens. Cryptic TCF3(E2A)-PBX1 rearrangements were investigated using interphase fluorescence in situ hybridization. Results: Six (12%) of 52 B-lymphoblastic leukemias demonstrated BCL6 protein expression, with B-cell lymphoblastic leukemias containing a t(1;19,) translocation demonstrating the strongest staining (three of three). Additional t(1;19) cases beyond the screening study showed similar results. Public microarray expression database mining showed that BCL6 messenger RNA expression levels in B-lymphoblastic leukemia correlated with the protein expression findings. Finally, other markers of B-cell development correlated with BCL6 expression in t(1;19) B-lymphoblastic leukemia cases, with LMO2 and HGAL proteins expressed in six (67%) of nine and eight (89%) of nine cases, respectively. Conclusions: BCL6 expression is present in a subset of B-lymphoblastic leukemias, especially in cases containing the 1;19 translocation. Investigation for TCF3(E2A)-PBX1 rearrangements may be useful in BCL6-positive B-lymphoblastic leukemia. (C) American Society for Clinical Pathology

引用

页码：547 / 557

页数：11

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