Identification of hotspots for NO and N2O production and consumption in counter- and co-diffusion biofilms for simultaneous nitrification and denitrification

A membrane-aerated biofilm reactor (MABR) provides a counter-current substrate diffusion geometry in which oxygen is supplied from a gas-permeable membrane on which a biofilm is grown. This study hypothesized that an MABR would mitigate NO and N2O emissions compared with those from a conventional biofilm reactor (CBR). Two laboratory-scale reactors, representing an MABR and CBR, were operated by feeding synthetic industrial wastewater. The surficial nitrogen removal rate for the MABR [4.51 +/- 0.52 g-N/(m(2) day)] was higher than that for the CBR [3.56 +/- 0.81 g-N/(m(2) day)] (p < 0.05). The abundance of beta-proteobacterial ammonia-oxidizing bacteria in the MABR biofilm aerobic zone was high. The NO and N2O concentrations at the biofilm-liquid interface in the MABR were 0.0066 +/- 0.0014 and 0.01 +/- 0.0009 mg-N/L, respectively, two and 28 times lower than those in the CBR. The NO and N2O production hotspots were closely located in the MABR aerobic zone.