Evaluation of modified vaccinia virus Ankara based recombinant SARS vaccine in ferrets

被引：138

作者：

Czub, M

Weingartl, H

Czub, S

He, RT

Cao, JX

机构：

[1] Canadian Sci Ctr Human & Anim Hlth, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

[2] Canadian Sci Ctr Human & Anim Hlth, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3R2, Canada

[3] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB R3T 2N2, Canada

来源：

VACCINE | 2005年 / 23卷 / 17-18期

关键词：

vaccinia virus; SARS; ferrets; vaccine; hepatitis;

D O I：

10.1016/j.vaccine.2005.01.033

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) remains a threat to cause epidemics as evidenced by recent sporadic cases in China. In this communication, we evaluated the efficacy and safety of two SARS vaccine candidates based on the recombinant modified vaccinia Ankara (MVA) expressing SARS-CoV spike or nucleocapsid proteins in ferrets. No clinical signs were observed in all the ferrets challenged with SARS-CoV. On the other hand, vaccination did not prevent SARS-CoV infection in ferrets. In contrast, immunized ferrets (particularly those immunized with rMVA-spike) exhibited significantly stronger inflammatory responses and focal necrosis in liver tissue after SARS-CoV challenge than control animals. Thus, our data suggest that enhanced hepatitis is linked to vaccination with rMVA expressing SARS-CoV antigens. Crown Copyright (c) 2005 Published by Elsevier Ltd. All rights reserved.

引用

页码：2273 / 2279

页数：7

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