Ganglioside GM2 is substrate for a sialidase in MDCK cells

被引：3

作者：

Marchesini, S ^{[1
]}

Benaglia, G ^{[1
]}

Piccinotti, A ^{[1
]}

Bresciani, R ^{[1
]}

Preti, A ^{[1
]}

机构：

[1] Univ Brescia, Dept Biomed Sci & Biotechnol, I-25123 Brescia, Italy

来源：

FEBS LETTERS | 1998年 / 428卷 / 1-2期

关键词：

Madin-Darby canine kidney cell; asialo-monosialoganglioside; 2; sialidase; fluorescent ganglioside; N-lissamine rhodamine;

D O I：

10.1016/S0014-5793(98)00510-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent CMI remained essentially undegraded, indicating a catabolic processing at lysosomal level. (C) 1998 Federation of European Biochemical Societies.

引用

页码：115 / 117

页数：3

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