Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors

被引：7

作者：

Abi, Akram ^{[1
]}

Hartig, Dave ^{[2
]}

Vorlaender, Karl ^{[2
]}

Wang, Anqi ^{[1
]}

Scholl, Stephan ^{[2
]}

Joerdening, Hans-Joachim ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Tech Chem, Gaussstr 17, D-38106 Braunschweig, Germany

[2] Tech Univ Carolo Wilhelmina Braunschweig, Inst Chem & Thermal Proc Engn, Braunschweig, Germany

来源：

ENGINEERING IN LIFE SCIENCES | 2019年 / 19卷 / 01期

关键词：

Adsorption; Continuous packed bed reactor; Downstream processing; Laminaribiose; Zeolite BEA; ISOMALTOSE; SEPARATION; PHASE;

D O I：

10.1002/elsc.201800110

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g(Laminaribiose) g(sugar)(-1) in the desorbate with a total productivity of 5.6 mg(Laminaribiose) L-enzyme bed(-1) h(-1) without the use of recycles.

引用

页码：4 / 12

页数：9

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