Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with fluorescent voltage sensor in rat

被引：14

作者：

Ahrens, Kurt F. ^{[1
]}

Heider, Barbara ^{[1
]}

Lee, Hanson ^{[2
]}

Isacoff, Ehud Y. ^{[2
]}

Siegel, RalphM. ^{[1
]}

机构：

[1] Rutgers State Univ, Ctr Mol & Behav Neurosci, Newark, NJ 07102 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

FRONTIERS IN NEURAL CIRCUITS | 2012年 / 6卷

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

single cell; cortex; two-photon imaging; genetic engineering; vision; ADENOASSOCIATED VIRUS VECTORS; VISUAL ASSOCIATION CORTEX; NEURAL ACTIVITY; FUNCTIONAL ARCHITECTURE; MULTIPHOTON MICROSCOPY; CALCIUM INDICATORS; MAMMALIAN-CELLS; BEHAVING MONKEY; GENE-EXPRESSION; SINDBIS VECTOR;

D O I：

10.3389/fncir.2012.00015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A fluorescent voltage sensor protein "Flare" was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular region of visual cortex in rat. Injection sites were selected based on intrinsic optical imaging. Expression of Flare occurred in the cell bodies and dendritic processes. Neurons imaged in vivo using two-photon scanning microscopy typically revealed the soma best, discernable against the background labeling of the neuropil. Somatic fluorescence changes were correlated with flashed visual stimuli, however, averaging was essential to observe these changes. This study demonstrates that the genetic modification of single neurons to express a fluorescent voltage sensor can be used to assess neuronal activity in vivo.

引用

页数：9

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