Clinical Usefulness of Novel Immunochromatographic Detection Device for Porphyromonas gingivalis in Evaluating Effects of Scaling and Root Planing and Local Antimicrobial Therapy

被引：7

作者：

Nakayama, Yohei ^{[1
,2
]}

Ogata, Yorimasa ^{[1
,2
]}

Hiromatsu, Yuki ^{[1
,2
]}

Imamura, Kentaro ^{[3
]}

Suzuki, Eiichi ^{[3
]}

Saito, Atsushi ^{[3
]}

Shirakawa, Satoshi ^{[4
]}

Nagano, Takatoshi ^{[4
]}

Gomi, Kazuhiro ^{[4
]}

Morozumi, Toshiya ^{[5
]}

Watanabe, Kaori ^{[6
]}

Akiishi, Kazuhiro ^{[7
]}

Yoshie, Hiromasa ^{[5
]}

机构：

[1] Nihon Univ, Sch Dent, Dept Periodontol, Matsudo, Chiba, Japan

[2] Nihon Univ, Sch Dent, Res Inst Oral Sci, Matsudo, Chiba, Japan

[3] Tokyo Dent Coll, Dept Periodontol, Tokyo, Japan

[4] Tsurumi Univ, Sch Dent Med, Dept Periodontol, Yokohama, Kanagawa, Japan

[5] Niigata Univ, Div Periodontol, Dept Oral Biol Sci, Grad Sch Med & Dent Sci, Niigata, Japan

[6] Showa Yakuhin Kako, Res Inst, Tokyo, Japan

[7] Denka Seiken, Reagent Res & Dev Dept, Gosen, Niigata, Japan

来源：

JOURNAL OF PERIODONTOLOGY | 2016年 / 87卷 / 10期

关键词：

Bacteria; drug delivery systems; immunochromatography; periodontitis; Porphyromonas gingivalis; NONSURGICAL PERIODONTAL THERAPY; REAL-TIME PCR; SUBGINGIVAL PLAQUE; TREPONEMA-DENTICOLA; QUANTIFICATION; ACTINOMYCETEMCOMITANS; ANTIBODIES; PROTECTS; BACTERIA; DISEASES;

D O I：

10.1902/jop.2016.160147

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: The authors have previously reported development of a novel immunochromatographic device (DK13-PG-001) for specific detection of Porphyromonas gingivalis (Pg). In this study, clinical usefulness of the detection device during periodontal therapy is presented. Methods: The multicenter study was conducted with 62 patients contributing 118 periodontitis sites with probing depth (PD) of 4 to 9 mm. Subgingival plaque samples were used for detection of Pg by DK13-PG-001 and the PCR-invader method at: 1) baseline (BL); 2) reevaluation (RE; after scaling and root planing); and 3) final evaluation (FE; after local drug delivery system). Periodontal examinations were performed concurrently with the test for Pg detection. Plasma immunoglobulin G (IgG) titers against Pg were also determined in patients using an enzyme-linked immunosorbent assay. Results: DK13-PG-001 score and number of Pg by the PCR-invader method showed a strong correlation (r = 0.862) at three stages during periodontal therapy (n = 354). High sensitivity and specificity of DK13-PG-001, in comparison with the PCR-invader method, were shown. A significant correlation was found among device score, number of Pg by the PCR-invader method, and PD and clinical attachment level at BL and RE. Plasma IgG titers against Pg were significantly reduced at FE in comparison with BL. Weak but significant correlations between IgG titers and device scores were shown at BL and FE. Conclusion: Results suggest the DK13-PG-001 device is a useful tool for detection of Pg in dental offices and can aid clinical evaluation of the extent of periodontitis and therapeutic efficacy.

引用

页码：1238 / 1247

页数：10

共 40 条

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