Human limbal epithelial stem cell regulation, bioengineering and function

被引：80

作者：

Bonnet, Clemence ^{[1
,2
]}

Gonzalez, Sheyla ^{[1
]}

Roberts, JoAnn S. ^{[1
]}

Robertson, Sarah Y. T. ^{[1
]}

Ruiz, Maxime ^{[1
]}

Zheng, Jie ^{[3
]}

Deng, Sophie X. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Stein Eye Inst, Cornea Div, Los Angeles, CA 90095 USA

[2] Univ Paris, Cochin Hosp, AP HP, Cornea Dept, F-75014 Paris, France

[3] Univ Calif Los Angeles, Stein Eye Inst, Div Basic Sci, Los Angeles, CA 90095 USA

来源：

PROGRESS IN RETINAL AND EYE RESEARCH | 2021年 / 85卷

关键词：

Limbal stem cell; Limbal stem cell deficiency; Wnt signaling pathway; Notch signaling pathway; Cell therapy; In vivo laser scanning confocal microscopy; Anterior segment coherence tomography; Small molecules; VIVO CONFOCAL MICROSCOPY; OPTICAL COHERENCE TOMOGRAPHY; WNT SIGNALING PATHWAY; CORNEO-SCLERAL LIMBUS; SIDE-POPULATION CELLS; STEM/PROGENITOR CELLS; IMPRESSION CYTOLOGY; BETA-CATENIN; NICHE CELLS; AMNIOTIC MEMBRANE;

D O I：

10.1016/j.preteyeres.2021.100956

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The corneal epithelium is continuously renewed by limbal stem/progenitor cells (LSCs), a cell population harbored in a highly regulated niche located at the limbus. Dysfunction and/or loss of LSCs and their niche cause limbal stem cell deficiency (LSCD), a disease that is marked by invasion of conjunctival epithelium into the cornea and results in failure of epithelial wound healing. Corneal opacity, pain, loss of vision, and blindness are the consequences of LSCD. Successful treatment of LSCD depends on accurate diagnosis and staging of the disease and requires restoration of functional LSCs and their niche. This review highlights the major advances in the identification of potential LSC biomarkers and components of the LSC niche, understanding of LSC regulation, methods and regulatory standards in bioengineering of LSCs, and diagnosis and staging of LSCD. Overall, this review presents key points for researchers and clinicians alike to consider in deepening the understanding of LSC biology and improving LSCD therapies.

引用

页数：27

共 235 条

[1] Small-molecule inhibition of Wnt signaling abrogates dexamethasone-induced phenotype of primary human trabecular meshwork cells [J].