Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium: Involvement of versatile monooxygenase

被引：57

作者：

Hirosue, Shinji ^{[1
,2
]}

Tazaki, Masahiro ^{[1
]}

Hiratsuka, Nobuhiro ^{[1
]}

Yanai, Satoshi ^{[2
]}

Kabumoto, Hiroki ^{[2
]}

Shinkyo, Raku ^{[3
]}

Arisawa, Akira ^{[2
]}

Sakaki, Toshiyuki ^{[3
]}

Tsunekawa, Hiroshi ^{[2
]}

Johdo, Osamu ^{[2
]}

Ichinose, Hirofumi ^{[1
]}

Wariishi, Hiroyuki ^{[1
,4
,5
]}

机构：

[1] Kyushu Univ, Fac Agr, Higashi Ku, Fukuoka 8128581, Japan

[2] Mercian Corp, Bioresource Labs, Iwata 4380078, Japan

[3] Toyama Prefectural Univ, Fac Engn, Dept Biotechnol, Toyama 9390398, Japan

[4] Kyushu Univ, Bioarchitecture Ctr, Higashi Ku, Fukuoka 8128581, Japan

[5] Kyushu Univ, Innovat Ctr Med Redox Nav, Higashi Ku, Fukuoka 8128581, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 407卷 / 01期

关键词：

Cytochrome P450; White-rot basidiomycete; Functional screening; Versatile functions; TRAMETES-VERSICOLOR; FUNGUS; LIGNIN; DEGRADATION; OXIDATION; PRODUCTS; DEPOLYMERIZATION; BIODEGRADATION; IDENTIFICATION; TRITERPENOIDS;

D O I：

10.1016/j.bbrc.2011.02.121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：118 / 123

页数：6

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