Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis

被引：133

作者：

Chung, Yuhee ^{[1
]}

Maharjan, Puna M. ^{[1
]}

Lee, Oksun ^{[1
]}

Fujioka, Shozo ^{[2
]}

Jang, Suyoun ^{[1
]}

Kim, Bokyung ^{[1
]}

Takatsuto, Suguru ^{[3
]}

Tsujimoto, Masafumi ^{[2
]}

Kim, Hobang ^{[1
,7
]}

Cho, Seoae ^{[4
]}

Park, Taesung ^{[4
,5
]}

Cho, Hyunwoo ^{[6
]}

Hwang, Ildoo ^{[6
]}

Choe, Sunghwa ^{[1
,7
]}

机构：

[1] Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Seoul 151747, South Korea

[2] RIKEN, Adv Sci Inst, Wako, Saitama 3510198, Japan

[3] Joetsu Univ Educ, Dept Chem, Joetsu, Niigata 9438512, Japan

[4] Seoul Natl Univ, Coll Nat Sci, Interdisciplinary Program Bioinformat, Seoul 151747, South Korea

[5] Seoul Natl Univ, Coll Nat Sci, Dept Stat, Seoul 151747, South Korea

[6] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea

[7] Seoul Natl Univ, Plant Genom & Breeding Inst, Seoul 151921, South Korea

来源：

PLANT JOURNAL | 2011年 / 66卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

brassinosteroids; gene expression; auxin; plant hormonal interaction; GUS; DWARF4; BOX PROTEIN TIR1; GENE-EXPRESSION; AUX/IAA PROTEINS; CPD GENE; GROWTH; ENCODES; TRANSCRIPTION; PATHWAY; BZR1; HOMEOSTASIS;

D O I：

10.1111/j.1365-313X.2011.04513.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>Brassinosteroids (BRs) are growth-promoting steroidal hormones. Despite the importance of BRs in plant biology, the signal that initiates BR biosynthesis remains unknown. Among the enzymes involved in BR biosynthesis in Arabidopsis (Arabidopsis thaliana), DWARF4 catalyzes the rate-determining step. Through both the histochemical analysis of DWF4pro:GUS plants and the direct measurement of endogenous BR content, we discovered that BR biosynthesis is stimulated by auxin. When DWF4pro:GUS was subjected to auxin dose-response tests and a time-course analysis, GUS activity started to increase at an auxin concentration of 10 nm, rising noticeably after 1 h of auxin treatment. In addition, the analysis of the DWF4pro:GUS line in BR- and auxin-mutant backgrounds revealed that the induction by auxin requires auxin-signaling pathways but not BRs, which implies that auxin signaling directly controls BR biosynthesis. Furthermore, chromatin immunoprecipitation assays confirmed that auxin inhibits the binding of the transcriptional repressor, BZR1, to the DWF4 promoter. A microarray analysis that was designed to examine the transcriptomes after treatment with auxin alone or auxin plus brassinazole (a BR biosynthetic inhibitor) revealed that genes previously characterized as being auxin responsive are not properly regulated when BR biosynthesis is disrupted by brassinazole. Therefore, our results support the idea that auxin regulates BR biosynthesis, and that auxin thus relies on synthesized BRs for some of its growth-promoting effects in Arabidopsis.

引用

页码：564 / 578

页数：15

共 60 条

[1] Early genes and auxin action [J].

Abel, S ;

Theologis, A .

PLANT PHYSIOLOGY, 1996, 111 (01) :9-17

[2] Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-κB and β-amyloid precursor protein [J].

Baek, SH ;

Ohgi, KA ;

Rose, DW ;

Koo, EH ;

Glass, CK ;

Rosenfeld, MG .

CELL, 2002, 110 (01) :55-67

[3] Hormone interactions and regulation of PsPK2:GUS compared with DR5:GUS and PID:GUS in Arabidopsis thaliana [J].

Bai, Fang ;

Demason, Darleen A. .

AMERICAN JOURNAL OF BOTANY, 2008, 95 (02) :133-145

[4] Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in arabidopsis [J].

Bancos, S ;

Szatmari, AM ;

Castle, J ;

Kozma-Bognar, L ;

Shibata, K ;

Yokota, T ;

Bishop, GJ ;

Nagy, F ;

Szekeres, M .

PLANT PHYSIOLOGY, 2006, 141 (01) :299-309

[5] Brassinosteroids interact with auxin to promote lateral root development in Arabidopsis [J].

Bao, F ;

Shen, JJ ;

Brady, SR ;

Muday, GK ;

Asami, T ;

Yang, ZB .

PLANT PHYSIOLOGY, 2004, 134 (04) :1624-1631

[6] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[7] Auxin signals - turning genes on and turning cells around [J].

Berleth, T ;

Krogan, NT ;

Scarpella, E .

CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (05) :553-563

[8] Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter [J].

Blázquez, MA ;

Green, R ;

Nilsson, O ;

Sussman, MR ;

Weigel, D .

PLANT CELL, 1998, 10 (05) :791-800

[9] A comparison of normalization methods for high density oligonucleotide array data based on variance and bias [J].

Bolstad, BM ;

Irizarry, RA ;

Åstrand, M ;

Speed, TP .

BIOINFORMATICS, 2003, 19 (02) :185-193

[10] BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis [J].

Caño-Delgado, A ;

Yin, YH ;

Yu, C ;

Vafeados, D ;

Mora-García, S ;

Cheng, JC ;

Nam, KH ;

Li, JM ;

Chory, J .

DEVELOPMENT, 2004, 131 (21) :5341-5351

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