A mitochondria-targeted fluorescent probe for fast detecting hypochlorite in living cells

被引：50

作者：

Li, Hongfang ^{[1
]}

Miao, Yu ^{[1
]}

Liu, Zhixue ^{[1
]}

Wu, Xue ^{[1
]}

Piao, Chunxiang ^{[2
]}

Zhou, Xin ^{[1
,3
]}

机构：

[1] Yanbian Univ, Dept Chem, Res Ctr Chem Biol, Yanji 133002, Peoples R China

[2] Yanbian Univ, Coll Agr, Food Res Ctr, Yanji 133002, Peoples R China

[3] Qingdao Univ, Dept Chem, Qingdao 266071, Peoples R China

来源：

DYES AND PIGMENTS | 2020年 / 176卷

基金：

中国博士后科学基金;

关键词：

Fluorescent probe; Hypochiorite; Cell imaging; PET; REACTIVE OXYGEN; LUMINESCENT PROBES; OXIDATIVE STRESS; REDOX CYCLE; SE-BODIPY; ACID; WATER; HOCL; STRATEGY;

D O I：

10.1016/j.dyepig.2020.108192

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fast-responsive fluorescent probe PI-Py was designed and synthesized for sensitive and selective detecting hypochlorite (ClO-). The probe PI-Py was intrinsically non-fluorescent due to the PET effect of the phenothiazine moiety. After reacting with ClO-, the PET effect was blocked, and a strong green emission of the BODIPY unit was recovered. The probe PI-Py showed an excellent selectivity towards ClO- in comparison of other ROS. The limit of detection for ClO- was calculated to be 1.7 nM, proving the probe's high sensitivity. Moreover, the probe PI-Py has been proved to be effective in visualizing the variations of exogenous ClO- in living HeLa cells. A colocalization study employing Mito Tracker red reveals that the PI-Py is specifically located in the mitochondria.

引用

页数：5

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