Genomic insights into a new acidophilic, copper-resistant Desulfosporosinus isolate from the oxidized tailings area of an abandoned gold mine

被引:39
作者
Mardanov, Andrey V. [1 ]
Panova, Inna A. [2 ]
Beletsky, Alexey V. [1 ]
Avakyan, Marat R. [2 ]
Kadnikov, Vitaly V. [1 ]
Antsiferov, Dmitry V. [2 ]
Banks, David [4 ,5 ]
Frank, Yulia A. [2 ]
Pimenov, Nikolay V. [3 ]
Ravin, Nikolai V. [1 ]
Karnachuk, Olga V. [2 ]
机构
[1] Russian Acad Sci, Biotechnol Res Ctr, Inst Bioengn, Prosp 60 Let Oktiabria,Bldg 7-1, Moscow 119071, Russia
[2] Tomsk State Univ, Lab Biochem & Mol Biol, Lenina Prospect 36, Tomsk 634050, Russia
[3] Russian Acad Sci, Biotechnol Res Ctr, Winogradsky Inst Microbiol, Pr 60 Letiya Oktyabrya 7-2, Moscow 119071, Russia
[4] Univ Glasgow, Sch Engn Syst Power & Energy, James Watt Bldg South, Glasgow G12 8QQ, Lanark, Scotland
[5] Holymoor Consultancy Ltd, 360 Ashgate Rd, Chesterfield S40 4BW, Derby, England
关键词
acidophilic sulfate-reducing bacteria; metal resistance; polyphosphates; Desulfosporosinus; SULFATE-REDUCING BACTERIA; MICROBIAL COMMUNITIES; LOW PH; GEOCHEMICAL DYNAMICS; URANIUM REDUCTION; SP-NOV; METAL; SULFUR; SEDIMENTS; DRAINAGE;
D O I
10.1093/femsec/fiw111
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbial sulfate reduction in acid mine drainage is still considered to be confined to anoxic conditions, although several reports have shown that sulfate-reducing bacteria occur under microaerophilic or aerobic conditions. We have measured sulfate reduction rates of up to 60 nmol S cm(-3) day(-1) in oxidized layers of gold mine tailings in Kuzbass (SW Siberia). A novel, acidophilic, copper-tolerant Desulfosporosinus sp. I2 was isolated from the same sample and its genome was sequenced. The genomic analysis and physiological data indicate the involvement of transporters and additional mechanisms to tolerate metals, such as sequestration by polyphosphates. Desulfosporinus sp. I2 encodes systems for a metabolically versatile life style. The genome possessed a complete Embden-Meyerhof pathway for glycolysis and gluconeogenesis. Complete oxidation of organic substrates could be enabled by the complete TCA cycle. Genomic analysis found all major components of the electron transfer chain necessary for energy generation via oxidative phosphorylation. Autotrophic CO2 fixation could be performed through theWood-Ljungdahl pathway. Multiple oxygen detoxification systems were identified in the genome. Taking into account the metabolic activity and genomic analysis, the traits of the novel isolate broaden our understanding of active sulfate reduction and associated metabolism beyond strictly anaerobic niches.
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页数:14
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