Dendronized Chitosan Derivative as a Biocompatible Gene Delivery Carrier

被引：65

作者：

Deng, Junjie ^{[2
,3
]}

Zhou, Yanfang ^{[1
,4
]}

Xu, Bo ^{[1
]}

Mai, Kaijin ^{[2
,3
]}

Deng, Yubin ^{[1
]}

Zhang, Li-Ming ^{[2
,3
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Pathophysiol, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, DSAPM Lab, Guangzhou 510275, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, PCFM Lab, Inst Polymer Sci, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China

[4] Guangdong Med Coll, Dept Pathophysiol, Dongguan 323808, Peoples R China

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 03期

基金：

中国国家自然科学基金;

关键词：

IN-VITRO; TRANSFECTION EFFICIENCY; CHEMICAL-MODIFICATION; PAMAM DENDRIMERS; CHEMISTRY; COPOLYMER; OLIGOMERS; POLYMERS; DESIGN; HYBRID;

D O I：

10.1021/bm101303f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve the transfection efficiency of chitosan as a nonviral gene delivery vector, a dendronized chitosan derivative was prepared by a copper-catalyzed azide alkyne cyclization reaction of propargyl focal point poly(amidoamine) dendron with 6-azido-6-deoxy-chitosan. Its structure was characterized by H-1 NMR and FTIR analyses and its buffering capacity was evaluated by acid-base titration. In particular, its complexation with plasmid DNA was investigated by agarose gel electrophoresis, zeta potential, and particle size analyses as well as transmission electron microscopy observation. Compared to unmodified chitosan, such a chitosan derivative has better water solubility;rid buffering capacity. Compared to commonly used polyethyleneimine (PEI, 25 kDa), it could exhibit enhanced transfection efficiency in some cases and lower cell toxicity, as confirmed by in vitro transfection and cytotoxicity tests in human kidney 293T and human nasopharyngeal carcinoma CNE2 cell lines, In addition, the effect of serum on its transfection efficiency was also studied.

引用

页码：642 / 649

页数：8

共 40 条

[21] Structural determination and interior polarity of self-aggregates prepared from deoxycholic acid-modified chitosan in water [J].