Isolation and multilocus genotyping of Toxoplasma gondii in seronegative rodents in Brazil

被引：27

作者：

Araujo, Jacqueline B.

da Silva, Aristeu V. ^{[1
,3
]}

Rosa, Ronaldo C. ^{[2
]}

Mattei, Rodrigo J. ^{[2
]}

da Silva, Rodrigo C. ^{[3
]}

Richini-Pereira, Virginia B. ^{[3
]}

Langoni, Helio ^{[3
]}

机构：

[1] Univ Estadual Feira de Santana, Lab Anal Clin & Parasitol, Dept Ciencias Biol, UEFS, BR-44036900 Feira De Santana, BA, Brazil

[2] Univ Paranaense, Curso Grad Med Vet, BR-87502210 Umuarama, Parana, Brazil

[3] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Sao Paulo, Brazil

来源：

VETERINARY PARASITOLOGY | 2010年 / 174卷 / 3-4期

关键词：

Toxoplasmosis; Mus musculus; Rattus rattus; Antibody detection; Bioassay; Genotyping; RATS RATTUS-NORVEGICUS; IDENTIFICATION; INFECTION; CATS; PCR;

D O I：

10.1016/j.vetpar.2010.08.039

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Synanthropic rodents, mainly rats and mice, become ecologically associated with men due to changes in their ecosystems caused by human activities. These animals may take part in the epidemiological cycles of several diseases, including toxoplasmosis. The presence of serum antibodies to Toxoplasma gondii in 43 rodents captured in the urban area of Umuarama, PR, Brazil, was verified by modified agglutination test (MAT). Brain and heart samples were also collected and bioassayed in mice for the isolation of the parasite. Isolated samples were analyzed by 12 multilocus genotyping. Although all rodents were seronegative, the parasite was isolated in one mouse (Mus musculus) and one rat (Rattus rattus). Genotyping showed that these samples were similar to those previously isolated from cats in the state of Parana, Brazil. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：328 / 331

页数：4

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