Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes

被引：17
作者：
Liu, Jianguo
Lu, Junren
Zhao, Xiubo
Lu, Jianren
Cui, Zhanfeng
机构：
[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
[2] China Univ Petr, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China
[3] Univ Manchester, Sch Phys, Manchester M60 1QD, Lancs, England
来源：
JOURNAL OF MEMBRANE SCIENCE | 2007年 / 299卷 / 1-2期
关键词：
ultrafiltration; membrane; protein fractionation; glucose oxidase; catalase;
D O I：
10.1016/j.memsci.2007.04.044
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：222 / 228
页数：7
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