Enhancement of Neurite Outgrowth in Neuronal-Like Cells following Boron Nitride Nanotube-Mediated Stimulation

被引：202
作者：
Ciofani, Gianni [1 ]
Danti, Serena [2 ]
D'Alessandro, Delfo [2 ,3 ]
Ricotti, Leonardo [4 ]
Moscato, Stefania [3 ]
Bertoni, Giovanni [5 ]
Falqui, Andrea [5 ]
Berrettini, Stefano [2 ]
Petrini, Mario [6 ]
Mattoli, Virgilio [1 ]
Menciassi, Arianna [1 ,4 ]
机构：
[1] Scuola Super Sant Anna, Italian Inst Technol, I-56025 Pisa, Italy
[2] Univ Pisa, Dept Neurosci, Otol Cochlear Implant Unit, I-56124 Pisa, Italy
[3] Univ Pisa, Dept Human Morphol & Appl Biol, I-56126 Pisa, Italy
[4] Scuola Super Sant Anna, CRIM Lab, I-56025 Pisa, Italy
[5] Italian Inst Technol, I-16163 Genoa, Italy
[6] Univ Pisa, Dept Oncol & Transplants, I-56126 Pisa, Italy
来源：
ACS NANO | 2010年 / 4卷 / 10期
关键词：
boron nitride nanotubes; PC12; cells; piezoelectric stimulation; neuronal regeneration; DEEP BRAIN-STIMULATION; CARBON NANOTUBES; ELECTRICAL-STIMULATION; NERVE REGENERATION; SCIATIC-NERVE; DEFORMATION; ELECTRODES; POLYMER; GROWTH; INJURY;
D O I：
10.1021/nn101985a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this paper, we propose an absolutely innovative technique for the electrical stimulation of cells, based on piezoelectric nanoparticles. Ultrasounds are used to impart mechanical stress to boron nitride nanotubes incubated with neuronal-like PC12 cells. By virtue of their piezoelectric properties, these nanotubes can polarize and convey electrical stimuli to the cells. PC12 stimulated with the present method exhibit neurite sprout 30% greater than the control cultures after 9 days of treatment.
引用
收藏
页码：6267 / 6277
页数：11
相关论文
共 58 条
[1]   Development of flexible arrays for in vivo neuronal recording and stimulation [J].
Adams, C ;
Mathieson, K ;
Gunning, D ;
Cunningham, W ;
Rahman, M ;
Morrison, JD ;
Prydderch, ML .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2005, 546 (1-2) :154-159
[2]   PIEZOELECTRIC QUIDANCE CHANNELS ENHANCE REGENERATION IN THE MOUSE SCIATIC-NERVE AFTER AXOTOMY [J].
AEBISCHER, P ;
VALENTINI, RF ;
DARIO, P ;
DOMENICI, C ;
GALLETTI, PM .
BRAIN RESEARCH, 1987, 436 (01) :165-168
[3]   Deep brain stimulation, vagal nerve stimulation and transcranial stimulation: An overview of stimulation parameters and neurotransmitter release [J].
Albert, G. C. ;
Cook, C. M. ;
Prato, F. S. ;
Thomas, A. W. .
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 2009, 33 (07) :1042-1060
[4]   Deformation-driven electrical transport of individual boron nitride nanotubes [J].
Bai, Xuedong ;
Golberg, Dmitri ;
Bando, Yoshio ;
Zhi, Chunyi ;
Tang, Chengchun ;
Mitome, Masanori ;
Kurashima, Keiji .
NANO LETTERS, 2007, 7 (03) :632-637
[5]   Applications of Carbon Nanotubes in Biotechnology and Biomedicine [J].
Bekyarova, Elena ;
Ni, Yingchun ;
Malarkey, Erik B. ;
Montana, Vedrana ;
McWilliams, Jared L. ;
Haddon, Robert C. ;
Parpura, Vladimir .
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (01) :3-17
[6]  
Cellot G, 2009, NAT NANOTECHNOL, V4, P126, DOI [10.1038/NNANO.2008.374, 10.1038/nnano.2008.374]
[7]   Aligned protein-polymer composite fibers enhance nerve regeneration: A potential tissue-engineering platform [J].
Chew, Sing Yian ;
Mi, Ruifa ;
Hoke, Ahmet ;
Leong, Kam W. .
ADVANCED FUNCTIONAL MATERIALS, 2007, 17 (08) :1288-1296
[8]   Cytocompatibility, Interactions, and Uptake of Polyethyleneimine-Coated Boron Nitride Nanotubes by Living Cells: Confirmation of Their Potential for Biomedical Applications [J].
Ciofani, Gianni ;
Raffa, Vittoria ;
Menciassi, Arianna ;
Cuschieri, Alfred .
BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (04) :850-858
[9]   Potential applications of boron nitride nanotubes as drug delivery systems [J].
Ciofani, Gianni .
EXPERT OPINION ON DRUG DELIVERY, 2010, 7 (08) :889-893
[10]   Assessing cytotoxicity of boron nitride nanotubes: Interference with the MTT assay [J].
Ciofani, Gianni ;
Danti, Serena ;
D'Alessandro, Delfo ;
Moscato, Stefania ;
Menciassi, Arianna .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 394 (02) :405-411
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