Detection of DNA hybridizations using solid-state nanopores

被引：33

作者：

Balagurusamy, Venkat S. K. ^{[1
]}

Weinger, Paul ^{[1
]}

Ling, Xinsheng Sean ^{[1
]}

机构：

[1] Brown Univ, Dept Phys, Providence, RI 02912 USA

来源：

NANOTECHNOLOGY | 2010年 / 21卷 / 33期

关键词：

POLYMER TRANSLOCATION; HAIRPIN MOLECULES; SINGLE; DISCRIMINATION; MEMBRANE; CHANNEL; ACID; PORE;

D O I：

10.1088/0957-4484/21/33/335102

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We report an experimental study of using DNA translocation through solid-state nanopores to detect the sequential arrangement of two double-stranded 12-mer hybridization segments on a single-stranded DNA molecule. The sample DNA is a trimer molecule formed by hybridizing three single-stranded oligonucleotides. A polystyrene bead is attached to the end of the trimer DNA, providing a mechanism in slowing down the translocation and suppressing the thermal diffusion, thereby allowing the detection of short features of DNA by standard patch-clamp electronics. The electrical signature of the translocation of a trimer molecule through a nanopore has been identified successfully in the temporal traces of ionic current. The results reported here represent the first successful attempt in using a solid-state nanopore as an ionic scanning device in resolving individual hybridization segments (or 'probes') on a DNA molecule.

引用

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