Inhibition of tumorigenesis by intratumoral delivery of the circadian gene mPer2 in C57BL/6 mice

被引：64

作者：

Hua, H.

Wang, Y.

Wan, C.

Liu, Y.

Zhu, B.

Wang, X.

Wang, Z. ^{[1
]}

Ding, J. M.

机构：

[1] Sichuan Univ, W China Med Ctr, State Key Lab Biotherapy, Hlth Minist Key Lab Chronobiol, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, W China Hosp, Mol Oncol Lab, Chengdu 610041, Sichuan, Peoples R China

[3] E Carolina Univ, Brody Sch Med, Dept Physiol, Greenville, NC USA

来源：

CANCER GENE THERAPY | 2007年 / 14卷 / 09期

关键词：

circadian clock genes; Period2; tumorigenesis;

D O I：

10.1038/sj.cgt.7701061

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biological clocks are intrinsic time-keeping systems that regulate behavior and physiological functions in most living organisms. Previous works suggested a possible link between the endogenous circadian clock and cell cycle regulation. The mammalian Period-2 gene ( mPer2), an important component of the circadian clock mechanism, is recently demonstrated to play an important role in repressing tumor growth. In this study, we found that polyethylenimine-mediated intratumoral Per2 gene delivery had significant antitumor effects in C57BL/6 mice transplanted with Lewis lung carcinoma. Our data illustrated that the Per2 gene delivery inhibited PCNA expression and induced apoptosis. Our results support the emerging role of the circadian clock in critical aspects of tumorigenesis. These findings underscore the potential use of Per2 gene delivery as a novel therapeutic intervention for the treatment of malignant tumors.

引用

页码：815 / 818

页数：4