A novel centrifugal impeller bioreactor .1. Fluid circulation, mixing, and liquid velocity profiles

被引:0
作者
Wang, SJ
Zhong, JJ
机构
[1] E CHINA UNIV SCI & TECHNOL,BIOCHEM ENGN RES INST,SHANGHAI 200237,PEOPLES R CHINA
[2] E CHINA UNIV SCI & TECHNOL,STATE KEY LAB BIOREACTOR ENGN,SHANGHAI 200237,PEOPLES R CHINA
关键词
bioreactor development; centrifugal impeller; fluid circulation; liquid velocity profile; mixing; shear stress;
D O I
10.1002/(SICI)1097-0290(19960905)51:5<511::AID-BIT2>3.0.CO;2-F
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A novel centrifugal impeller bioreactor for shear-sensitive biological systems was designed by installing a centrifugal-pumplike impeller in a stirred vessel. The fluid circulation, mixing, and liquid velocity profiles in the new bioreactor (5-L) were assessed as functions of the principal impeller designing and bioreactor operating parameters. The performances of the centrifugal impeller bioreactor were compared with those of a widely used cell-lift bioreactor. The newly developed bioreactor showed higher liquid lift capacity and shorter mixing time than the cell lift with com parable dimensions. Furthermore, the experiments of the liquid velocity profiles around an impeller region indicated that the centrifugal impeller bioreactor produced lower shear stress than the cell lift. This conclusion was also supported by evaluating the changes in size distributions of granulated agar particles that were sheared with those two types of impeller. (C) 1996 John Wiley & Sons, Inc.
引用
收藏
页码:511 / 519
页数:9
相关论文
共 20 条
  • [1] MASS TRANSFER AND MIXING RATES IN FERMENTATION VESSELS[J]. BLAKEBROUGH, N;SAMBAMURTHY, K. BIOTECHNOLOGY AND BIOENGINEERING, 1966(01)
  • [2] CALABRESE RV, 1989, CHEM ENG PROG, V85, P43
  • [3] MIXING OF VISCOELASTIC FLUIDS WITH HELICAL-RIBBON AGITATORS .1. MIXING TIME AND FLOW PATTERNS[J]. CARREAU, PJ;PATTERSON, I;YAP, CY. CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 1976(03)
  • [4] CHEN MH, 1986, PRINCIPLES CHEM ENG
  • [5] ANIMAL-CELL CULTURE IN STIRRED BIOREACTORS - OBSERVATIONS ON SCALE-UP[J]. CHISTI, Y. BIOPROCESS ENGINEERING, 1993(05)
  • [6] CHISTI Y, 1993, J CHEM TECHNOL BIOT, V58, P331
  • [7] VELOCITY PROFILES AND PUMPING CAPACITIES FOR TURBINE TYPE IMPELLERS[J]. COOPER, RG;WOLF, D. CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 1968(02)
  • [8] CULTIVATION OF PLANT-CELLS IN A STIRRED VESSEL - EFFECT OF IMPELLER DESIGN[J]. HOOKER, BS;LEE, JM;AN, GH. BIOTECHNOLOGY AND BIOENGINEERING, 1990(03)
  • [9] ANIMAL-CELL BIOREACTORS - RECENT ADVANCES AND CHALLENGES TO SCALE-UP[J]. HU, WS;PESHWA, MV. CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 1991(02)
  • [10] McCabe W.L., 1976, UNIT OPERATIONS CHEM