Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis

被引：2
作者：
Yan, J. -C.
Zhu, F. -H.
Wu, H. -L. [1 ]
机构：
[1] Key Lab Biot Environm & Ecol Safety, Bangbu, Anhui, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2011年 / 10卷 / 01期
关键词：
Pelophylax hubeiensis; Genetic marker; Microsatellite; Polymorphism; DISPERSAL;
D O I：
10.4238/vol10-1gmr1003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Twelve polymorphic microsatellite loci were isolated from an (AC)(n)- and (AG)(n)-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0.656, respectively. No significant linkage disequilibrium was detected among these loci. However, two significant deviations from HWE were discovered at loci Pehu-11 and Pehu-12 (P < 0.05). MICRO-CHECKER tests showed that null alleles could be present at locus Pehu-12. These polymorphic microsatellite loci can be employed for exploring mating mechanisms, population genetic structure and other relevant genetic investigations of P. hubeiensis.
引用
收藏
页码：268 / 272
页数：5
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