An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins

被引：31

作者：

Chien, Chih-Ta Henry ^{[1
]}

Helfinger, Lukas R. ^{[1
]}

Bostock, Mark J. ^{[1
]}

Solt, Andras ^{[1
]}

Tan, Yi Lei ^{[1
]}

Nietlispach, Daniel ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, 80 Tennis Court Rd, Cambridge CB2 1GA, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2017年 / 139卷 / 42期

基金：

英国生物技术与生命科学研究理事会;

关键词：

BETA(2)-ADRENERGIC RECEPTOR; NANODISCS; SPECTROSCOPY; ENVIRONMENT; BICELLES; DYNAMICS; SAPOSIN; LIPIDS;

D O I：

10.1021/jacs.7b06730

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Based on the saposin-A (SapA) scaffold protein, we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins (MPs) under close to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry, enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of MPs. Three representative MPs, including one G-protein-coupled receptor, were successfully incorporated into SapA-dimyristoylphosphatidylcholine nanoparticles and studied by solution NMR spectroscopy.

引用

页码：14829 / 14832

页数：4

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