Unconventional Salt Trend from Soft to Stiff in Single Neurofilament Biopolymers

被引：37

作者：

Beck, Roy ^{[1
,2
,6
]}

Deek, Joanna ^{[3
]}

Choi, Myung Chul ^{[1
,2
,6
]}

Ikawa, Taiji ^{[4
]}

Watanabe, Osamu ^{[4
]}

Frey, Erwin ^{[5
]}

Pincus, Philip ^{[1
,6
]}

Safinya, Cyrus R. ^{[1
,2
,6
]}

机构：

[1] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[4] Toyota Cent Res & Dev Labs Inc, Aichi 4801192, Japan

[5] Univ Munich, Dept Phys, Munich, Germany

[6] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

来源：

LANGMUIR | 2010年 / 26卷 / 24期

基金：

美国国家科学基金会;

关键词：

ELECTROSTATIC PERSISTENCE LENGTH; INTERMEDIATE-FILAMENTS; SEMIFLEXIBLE POLYMERS; AXONAL-TRANSPORT; IN-VITRO; DNA; BRUSH; POLYELECTROLYTE; NETWORKS; DISEASE;

D O I：

10.1021/la103655x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present persistence length measurements on neurofilaments (NFs), an intermediate filament with protruding side arms, of the neuronal cytoskeleton. Tapping mode atomic force microscopy enabled us to visualize and trace at subpixel resolution photoimmobilized NFs, assembled at various subunit protein ratios, thereby modifying the side-arm length and chain density charge distribution. We show that specific polyampholyte sequences of the side arms can form salt-switchable intrafilament attractions that compete with the net electrostatic and steric repulsion and can reduce the total persistence length by half. The results are in agreement with present X-ray and microscopy data yet present a theoretical challenge for polyampholyte interchain interactions.

引用

页码：18595 / 18599

页数：5

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