Mycobacterium avium uses apoptotic macrophages as tools for spreading

被引：59

作者：

Early, Julie ^{[1
,2
]}

Fischer, Kay ^{[1
]}

Bermudez, Luiz E. ^{[1
,2
,3
]}

机构：

[1] Oregon State Univ, Dept Biomed Sci, Coll Vet Med, Corvallis, OR 97331 USA

[2] Oregon State Univ, Program Mol & Cell Biol, Corvallis, OR 97331 USA

[3] Oregon State Univ, Dept Microbiol, Coll Sci, Corvallis, OR 97331 USA

来源：

MICROBIAL PATHOGENESIS | 2011年 / 50卷 / 02期

关键词：

Mycobacterium avium; Macrophages; Apoptosis; Spread; Escape; ARGININE TRANSLOCATION PATHWAY; PROGRAMMED CELL-DEATH; INFECTED MACROPHAGES; PSEUDOMONAS-SYRINGAE; PHAGOSOME MATURATION; BEIGE MICE; IFN-GAMMA; VIRULENCE; DISSEMINATION; TUBERCULOSIS;

D O I：

10.1016/j.micpath.2010.12.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Mycobacterium avium (MAC) lives and replicates in macrophages and causes disseminated disease in immunocompromised individuals. As a host response to control disease, many macrophages become apoptotic a few days after MAC infection. In this study, we hypothesized that MAC can survive autophagic and apoptotic macrophages and spread. Methods: Electron, time-lapse video, fluorescence microscopy. Apoptosis was determined by ELISA and TUNEL assays. Autophagy was seen by migration of LC3-1. Results: Apoptotic macrophages harbor chiefly viable MAC. MAC escapes both the vacuole and the macrophage once apoptosis is triggered, leaving the bacteria free to infect nearby macrophages in the process of spreading. In addition, some MAC species will have apoptotic bodies and are released in healthy macrophages following apoptotic body ingestion. Because autophagy precedes apoptosis, it was established that heat-killed MAC, and viable MAC induces autophagy in macrophages at similar rates, but MAC still survives. Conclusion: MAC spreading from cell-to-cell is triggered by the macrophage's attempt to kill the bacterium, undergoing apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：132 / 139

页数：8

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