A duplex PCR for differentiation of wild and vaccine virus of Aujeszky's disease

被引：4

作者：

Fonseca, A. A., Jr. ^{[1
]}

Dias, N. L. ^{[1
]}

Leite, R. C. ^{[2
]}

Heinemann, M. B. ^{[2
]}

Reis, J. K. P. ^{[2
]}

机构：

[1] MAPA, Lab Nacl Agropecuario, BR-33600000 Pedro Leopoldo, MG, Brazil

[2] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2010年 / 62卷 / 05期

关键词：

Aujeszky's disease; PCR; vaccine;

D O I：

10.1590/S0102-09352010000500032

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A duplex PCR was developed to differentiate the wild-type virus from the attenuated virus used in vaccinations. The PCR was able to amplify fragments of 493bp for glycoprotein E (gE) gene and 207bp for glycoprotein B (gB) gene. The analytical sensitivity was determined by addition of a virus field sample titled in the brain samples of pigs. The standard virus strain Shope, the vaccine strain Bartha, and ten other field isolates were subjected to PCR. The PCR was able to amplify fragments of gE and gB in all field samples and only fragments of gB were amplified in the attenuated virus, as expected. The technique was able to detect up to 10(0.5) TCID50/50 mu L virus in samples of brain. Duplex PCR proved to be an important tool for differentiation of naturally-infected animals and animals vaccinated with the virus deleted for gE.

引用

页码：1259 / 1262

页数：4

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