In-cell protein NMR and protein leakage

被引：56

作者：

Barnes, Christopher O.

Pielak, Gary J. ^{[1
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机构：

[1] Univ N Carolina, Dept Chem, Kenan Labs, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2011年 / 79卷 / 02期

关键词：

in-cell NMR; protein expression; protein leakage; RETRACTED ARTICLE. SEE; PERIPLASMIC PROTEIN; BACKBONE DYNAMICS; PG; 8206; VOLUME; SPECTROSCOPY; EXPRESSION; CYTOPLASM; DIFFUSION; HDEA;

D O I：

10.1002/prot.22906

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In-cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in-cell NMR experiments and show that leakage is likely when the protein being studied exceeds similar to 20% of the total cellular protein. Proteins 2011; 79:347-351. (C) 2010 Wiley-Liss, Inc.

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页码：347 / 351

页数：5

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