In vitro effects of Blepharocalyx salicifolius (HBK) O. Berg on the viability of Echinococcus ortleppi protoscoleces

被引：0

作者：

Noal, Charlise Bolson ^{[1
]}

Monteiro, Danieli Urach ^{[1
]}

de Brum, Thiele Faccim ^{[2
]}

Emmanouilidis, Jessica ^{[1
]}

Zanette, Regis Adriel ^{[3
]}

Morel, Ademir Farias ^{[4
]}

de Cassia Stefanon, Eliza Beti ^{[5
]}

Frosi, Marina ^{[5
]}

de la Rue, Mario Luiz ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Dept Microbiol & Parasitol, Ave Roraima,1000 Campus Univ,Predio 20,Sala 4226, BR-97105970 Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Farm Ind, Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Programa Posgrad Ciencias Biol Farmacol & Terapeu, Santa Maria, RS, Brazil

[4] Univ Fed Santa Maria, Dept Quim Organ, Santa Maria, RS, Brazil

[5] Ctr Univ Franciscano, Lab Farmacol Toxicol & Bot, Santa Maria, RS, Brazil

来源：

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 2017年 / 59卷

关键词：

Echinococcus ortleppi; Protoscolicidal; Scolicidal agent; Gallic acid; IDENTIFICATION; CATTLE; G1; G5;

D O I：

10.1590/S1678-9946201759042

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Scolicidal agents are important in the treatment of cystic echinococcosis. This study evaluated the scolicidal activity of the plant Blepharocalyx salicifolius (H.B.K.) Berg against Echinococcus ortleppi protoscoleces. The parasite species was identified by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (COX 1). B. salicifolius crude extract at concentrations of 100, 200, 300 and 400 mg/mL was analyzed at different times (5, 10, 15, 30, 45 and 60 min). N-butanol and ethyl acetate fractions (100 and 200 mg/mL) were also analyzed at 5, 10, 15 and 30 min. Both fractions showed 100% scolicidal activity at the concentration of 200 mg/mL at 5 min. Gallic acid, identified as the major compound of the ethyl acetate fraction- was responsible for the observed scolicidal activity. The results showed that crude extract and fractions of B. salicifolius have scolicidal effect against E. ortleppi protoscoleces.

引用

页数：5