Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14

被引：1
作者：
Perepelov, Andrei, V [1 ]
Li, Xiaodan [2 ]
Xu, Cong [2 ]
Filatov, Andrei, V [1 ]
Shashkov, Alexander S. [1 ]
Senchenkova, Sofia N. [1 ]
Liu, Bin [2 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow, Russia
[2] Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2019年 / 474卷
基金：
俄罗斯科学基金会; 中国国家自然科学基金;
关键词：
Vibrio cholerae; Bacterial polysaccharide structure; O-antigen; O-specific polysaccharide; Lipopolysaccharide; O-antigen gene cluster; GRASS; ESCHERICHIA-COLI; POLYSACCHARIDE; LIPOPOLYSACCHARIDE; STRAIN; CORE; O139;
D O I：
10.1016/j.carres.2019.01.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-polysaccharide (O-antigen) of Vibrio cholerae O14 was studied using chemical analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating unit of the O-antigen was established: where GlcpN(SHb) indicates 2-deoxy-2-[(S)-3-hydroxybutanoylamino]-D-glucose. We found that Vibrio cholerae O14 is similar to that of O-polysaccharide of Azospirillum brasilense S17, which has been reported earlier. Moreover, we predicted functions of all the genes in the O-antigen gene cluster according to the structure established. Our study enriches the existing O-antigen database of Vibrio cholerae, and further facilitates the bacterial serotype identification.
引用
收藏
页码：67 / 71
页数：5
相关论文
共 24 条
[1]  
ALBERT MJ, 1993, LANCET, V341, P704
[2]   Genetic Diversity of O-Antigen Biosynthesis Regions in Vibrio cholerae [J].
Aydanian, Antonina ;
Tang, Li ;
Morris, J. Glenn ;
Johnson, Judith A. ;
Stine, O. Colin .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (07) :2247-2253
[3]   GENESIS OF THE NOVEL EPIDEMIC VIBRIO-CHOLERAE-0139 STRAIN - EVIDENCE FOR HORIZONTAL TRANSFER OF GENES INVOLVED IN POLYSACCHARIDE SYNTHESIS [J].
BIK, EM ;
BUNSCHOTEN, AE ;
GOUW, RD ;
MOOI, FR .
EMBO JOURNAL, 1995, 14 (02) :209-216
[4]   The capsule polysaccharide structure and biogenesis for non-OI Vibrio cholerae NRT36S: genes are embedded in the LPS region [J].
Chen, Yuansha ;
Bystricky, Peter ;
Adeyeye, Jacob ;
Panigrahi, Pinaki ;
Ali, Afsar ;
Johnson, Judith A. ;
Bush, C. A. ;
Morris, J. G., Jr. ;
Stine, O. C. .
BMC MICROBIOLOGY, 2007, 7
[5]   Comparative genomic analysis of Vibrio cholerae:: Genes that correlate with cholera endemic and pandemic disease [J].
Dziejman, M ;
Balon, E ;
Boyd, D ;
Fraser, CM ;
Heidelberg, JF ;
Mekalanos, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1556-1561
[6]   Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17 [J].
Fedonenko, Yuliya P. ;
Konnova, Olga N. ;
Zdorovenko, Evelina L. ;
Konnova, Svetlana A. ;
Zatonsky, George V. ;
Shashkov, Alexander S. ;
Ignatov, Vladimir V. ;
Knirel, Yuriy A. .
CARBOHYDRATE RESEARCH, 2008, 343 (04) :810-816
[7]   Synthesis of the heteropolysaccharide O antigen of Escherichia coli O52 requires an ABC transporter:: Structural and genetic evidence [J].
Feng, L ;
Senchenkova, SN ;
Yang, JH ;
Shashkov, AS ;
Tao, J ;
Guo, HJ ;
Cheng, JS ;
Ren, Y ;
Knirel, YA ;
Reeves, PR ;
Wang, L .
JOURNAL OF BACTERIOLOGY, 2004, 186 (14) :4510-4519
[8]   COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA [J].
JANSSON, PE ;
KENNE, L ;
WIDMALM, G .
CARBOHYDRATE RESEARCH, 1989, 188 :169-191
[9]   STRUCTURAL STUDIES OF THE O-ANTIGEN POLYSACCHARIDES OF KLEBSIELLA O5 AND ESCHERICHIA-COLI O8 [J].
JANSSON, PE ;
LONNGREN, J ;
WIDMALM, G ;
LEONTEIN, K ;
SLETTENGREN, K ;
SVENSON, SB ;
WRANGSELL, G ;
DELL, A ;
TILLER, PR .
CARBOHYDRATE RESEARCH, 1985, 145 (01) :59-66
[10]   Interrupting Biosynthesis of O Antigen or the Lipopolysaccharide Core Produces Morphological Defects in Escherichia coli by Sequestering Undecaprenyl Phosphate [J].
Jorgenson, Matthew A. ;
Young, Kevin D. .
JOURNAL OF BACTERIOLOGY, 2016, 198 (22) :3070-3079
123