Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes

被引:14
作者
Qu, Xiaolu [1 ]
Wang, Qiannan [2 ]
Wang, Haiyan [1 ]
Huang, Shanjin [2 ]
机构
[1] Huazhong Agr Univ, Coll Hort & Forestry Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan, Peoples R China
[2] Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, Beijing 100084, Peoples R China
来源
BIO-PROTOCOL | 2020年 / 10卷 / 01期
基金
中国国家自然科学基金;
关键词
Arabidopsis; Pollen germination; Pollen grain; Pollen tube; Actin; Phalloidin staining; BINDING PROTEINS; GERMINATION; FILAMENTS; TURNOVER; GROWTH; POLYMERIZATION; DYNAMICS;
D O I
10.21769/BioProtoc.3509
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we describe the protocol of revealing and quantifying the spatial organization of actin using fluorescent phalloidin-staining in fixed Arabidopsis pollen grains and pollen tubes. We also introduce the method of assessing the stability and/or turnover rate of actin filaments in pollen cells using the treatment of latrunculin B.
引用
收藏
页数:10
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