Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes

被引：29

作者：

Liang, Xing-Wei ^{[1
]}

Ge, Zhao-Jia ^{[1
]}

Guo, Lei ^{[1
]}

Luo, Shi-Ming ^{[1
]}

Han, Zhi-Ming ^{[1
]}

Schatten, Heide ^{[2
]}

Sun, Qing-Yuan ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Reprod Biol, Inst Zool, Beijing 100101, Peoples R China

[2] Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA

来源：

FERTILITY AND STERILITY | 2011年 / 96卷 / 06期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; imprinted gene; oocyte; offspring; postovulatory oocyte aging; MOUSE OOCYTE; EXPRESSION; GROWTH; EMBRYO; GENES;

D O I：

10.1016/j.fertnstert.2011.09.022

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring. Design: Randomized research experimental study. Setting: Academic basic research laboratory. Animal(s): Mice. Intervention(s): Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring. Main Outcome Measure(s): Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19. Result(s): Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3. Conclusion(s): Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring. (Fertil Steril (R) 2011;96:1479-84. (C) 2011 by American Society for Reproductive Medicine.)

引用

页码：1479 / 1484

页数：6

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