Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA

被引：85

作者：

Brady, SF ^{[1
]}

Clardy, J ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 43期

关键词：

biosynthesis; DNA; heterologous expression; isocyanides; natural products;

D O I：

10.1002/anie.200501941

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) One gene, isnA, encodes the enzyme that converts the precursor amine functionality of tryptophan into the isocyanide moiety. The isolation and characterization of the isocyanide-containing environmental-DNA-derived antibiotic 1, its biosynthetic genes (isnA and isnB), and the general outline of the biosynthesis of 1 via intermediate 2 are presented (see scheme). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7063 / 7065

页数：3

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