Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell

被引:541
作者
Bastiaens, PIH [1 ]
Squire, A [1 ]
机构
[1] Imperial Canc Res Fund, Cell Biophys Lab, London WC2A 3PX, England
关键词
D O I
10.1016/S0962-8924(98)01410-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Flourescence lifetime imaging microscopy (FLIM) is a technique in which the mean fluorescence lifetime of a chromophore is measured at each spatially resolvable element of a microscope image. The nanosecond excited-state lifetime is independent of probe-concentration or light path length but dependent upon excited-state reactions such as fluorescence resonance energy transfer (FRET). These properties of fluorescence lifetimes allow exploration oft-he: molecular environment of labelled macromolecules in the interior of cells. Imaging of fluorescence lifetimes enables biochemical reactions to be followed at each microscopically resolvable location-within the cell.
引用
收藏
页码:48 / 52
页数:5
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