In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration

被引:10
作者
Bianchini, Matteo [1 ]
Giambelluca, Maria [2 ]
Scavuzzo, Maria Concetta [2 ]
Di Franco, Gregorio [1 ]
Guadagni, Simone [1 ]
Palmeri, Matteo [1 ]
Furbetta, Niccolo [1 ]
Gianardi, Desiree [1 ]
Costa, Aurelio [3 ]
Gentiluomo, Manuel [4 ]
Gaeta, Raffaele [5 ]
Pollina, Luca Emanuele [5 ]
Falcone, Alfredo [6 ]
Vivaldi, Caterina [6 ]
Di Candio, Giulio [1 ]
Biagioni, Francesca [7 ]
Busceti, Carla Letizia [7 ]
Soldani, Paola [2 ]
Puglisi-Allegra, Stefano [7 ]
Morelli, Luca [1 ,8 ]
Fornai, Francesco [2 ,7 ]
机构
[1] Univ Pisa, Dept Translat Res & New Technol Med & Surg, Gen Surg Unit, I-56124 Pisa, Italy
[2] Univ Pisa, Dept Translat Res & New Technol Med & Surg, Human Anat, I-56124 Pisa, Italy
[3] ASL Toscana Nord Ovest Pontedera Hosp, Gen Surg Unit, I-56025 Pontedera, Italy
[4] Univ Pisa, Dept Biol, I-56124 Pisa, Italy
[5] Univ Pisa, Dept Surg Mol Pathol & Crit Area, Div Surg Pathol, I-56124 Pisa, Italy
[6] Univ Pisa, Dept Translat Res & New Technol Med & Surg, Div Med Oncol, I-56124 Pisa, Italy
[7] IRCCS Neuromed Ist Neurol Mediterraneo, I-86077 Pozzilli, Italy
[8] Univ Pisa, EndoCAS Ctr Comp Assisted Surg, I-56124 Pisa, Italy
关键词
pancreatic ductal adenocarcinoma; alpha-synuclein; Western blotting; electron microscopy; neuroinvasion; electron-microscopy; ultrastructural stoichiometry; GAMMA-SYNUCLEIN; LEWY BODIES; DUCTAL ADENOCARCINOMA; PERINEURAL INVASION; PARKINSONS-DISEASE; PRION PROTEIN; CANCER; EXPRESSION; OVEREXPRESSION; AUTOPHAGY;
D O I
10.3390/ijms23073775
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
alpha-Synuclein (alpha-syn) is a protein involved in neuronal degeneration. However, the family of synucleins has recently been demonstrated to be involved in the mechanisms of oncogenesis by selectively accelerating cellular processes leading to cancer. Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers, with a specifically high neurotropism. The molecular bases of this biological behavior are currently poorly understood. Here, alpha-synuclein was analyzed concerning the protein expression in PDAC and the potential association with PDAC neurotropism. Tumor (PDAC) and extra-tumor (extra-PDAC) samples from 20 patients affected by PDAC following pancreatic resections were collected at the General Surgery Unit, University of Pisa. All patients were affected by moderately or poorly differentiated PDAC. The amount of alpha-syn was compared between tumor and extra-tumor specimen (sampled from non-affected neighboring pancreatic areas) by using in situ immuno-staining with peroxidase anti-alpha-syn immunohistochemistry, alpha-syn detection by using Western blotting, and electron microscopy by using alpha-syn-conjugated immuno-gold particles. All the methods consistently indicate that each PDAC sample possesses a higher amount of alpha-syn compared with extra-PDAC tissue. Moreover, the expression of alpha-syn was much higher in those PDAC samples from tumors with perineural infiltration compared with tumors without perineural infiltration.
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页数:20
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