Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

被引：13

作者：

Croxford, Matthew ^{[1
]}

Elbaum, Michael ^{[2
]}

Arigovindan, Muthuvel ^{[3
]}

Kam, Zvi ^{[4
]}

Agard, David ^{[5
]}

Villa, Elizabeth ^{[1
,6
]}

Sedat, John ^{[5
]}

机构：

[1] Univ Calif San Diego, Div Biol Sci, Sect Mol Biol, La Jolla, CA 92093 USA

[2] Weizmann Inst Sci, Dept Chem & Biol Phys, IL-760001 Rehovot, Israel

[3] Indian Inst Sci, Dept Elect Engn, Bengaluru 560012, India

[4] Weizmann Inst Sci, Dept Mol Cell Biol, IL-760001 Rehovot, Israel

[5] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA

[6] Univ Calif San Diego, HHMI, La Jolla, CA 92093 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 50期

关键词：

cryo-electron tomography; deconvolution; subtomogram analysis; structural biology; missing wedge; IN-SITU STRUCTURE; CRYOELECTRON TOMOGRAPHY; STRUCTURAL-ANALYSIS; RESOLUTION; VISUALIZATION; ABERRATIONS; CELLS;

D O I：

10.1073/pnas.2108738118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).

引用

页数：8

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