High frequency plant regeneration from nodal explants of Paulownia elongata

被引:15
|
作者
Ipekci, Z
Altinkut, A
Kazan, K
Bajrovic, K
Gozukirmizi, N
机构
[1] Res Inst Genet Engn & Biotechnol, Marmara Res Ctr, TUBITAK, TR-41470 Gebze Kocaeli, Turkey
[2] Univ Istanbul, Fac Sci, Vezneciler Istanbul, Turkey
[3] Univ Queensland, CSIRO, Plant Ind & Cooperat Res Ctr Trop Plant Protect, Brisbane, Qld 4072, Australia
关键词
Paulownia elongata; micropropagation; nodal explant; tissue culture;
D O I
10.1055/s-2001-12903
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
High frequency of plant regeneration from Paulownia elongate was obtained on Murashige and Skoog (MS) medium and Woody Plant Medium (WPM), with appropriate supplements of growth regulators. Leaves, leaves with petioles, internodes and nodes excised from 3-month-old non-aseptically grown P. elongata were used as explants. The highest shoot regeneration efficiency (93.7%) was obtained from the nodes of P. elongate on MS medium supplemented with 0.1 mg/ml naphthaleneacetic acid (NAB) and 1 mg/ml 6-benzylaminopurine (BAP). The highest root formation efficiency (100%) from the regenerated shoots was obtained on WPM supplemented with 1 mg/ml indolebutyric acid (IBA). Rooted plantlets were transplanted to soil with a survival efficiency of almost 100 %. The regeneration system reported here could be useful for rapid multiplication of elite genotypes of P. elongata in a short period of time.
引用
收藏
页码:113 / 115
页数:3
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