Protein structural deformation induced lifetime shortening of photosynthetic bacteria light-harvesting complex LH2 excited state

被引:42
作者
Chen, XH
Zhang, L
Weng, YX
Du, LC
Ye, MP
Yang, GZ
Fujii, R
Rondonuwu, FS
Koyama, Y
Wu, YS
Zhang, JP
机构
[1] Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100080, Peoples R China
[2] Kwansei Gakuin Univ, Fac Sci, Sanda, Hyogo 6691337, Japan
[3] Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
基金
日本学术振兴会;
关键词
D O I
10.1529/biophysj.104.053868
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Photosynthetic bacterial light-harvesting antenna complex LH2 was immobilized on the surface of TiO2 nanoparticles in the colloidal solution. The LH2/TiO2 assembly was investigated by the time-resolved spectroscopic methods. The excited-state lifetimes for carotenoid-containing and carotenoidless LH2 have been measured, showing a decrease in the excited-state lifetime of B850 when LH2 was immobilized on TiO2. The possibility that the decrease of the LH2 excited-state lifetime being caused by an interfacial electron transfer reaction between B850 and the TiO2 nanoparticle was precluded experimentally. We proposed that the observed change in the photophysical properties of LH2 when assembled onto TiO2 nanoparticles is arising from the interfacial-interaction-induced structural deformation of the LH2 complex deviating from an ellipse of less eccentric to a more eccentric ellipse, and the observed phenomenon can be accounted by an elliptical exciton model. Experiment by using photoinactive SiO2 nanoparticle in place of TiO2 and core complex LH1 instead of LH provide further evidence to the proposed mechanism.
引用
收藏
页码:4262 / 4273
页数:12
相关论文
共 50 条
[41]   Molecular model of LH1 light-harvesting complex of the photosynthetic center of Thermochromatium tepidum bacteria [J].
Khrenova M.G. ;
Polyakov I.V. ;
Grigorenko B.L. ;
Nemukhin A.V. .
Moscow University Chemistry Bulletin, 2013, 68 (2) :80-82
[42]   Spectroscopic studies of two spectral variants of light-harvesting complex 2 (LH2) from the photosynthetic purple sulfur bacterium Allochromatium vinosum [J].
Niedzwiedzki, Dariusz M. ;
Bina, David ;
Picken, Nichola ;
Honkanen, Suvi ;
Blankenship, Robert E. ;
Holten, Dewey ;
Cogdell, Richard J. .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2012, 1817 (09) :1576-1587
[43]   Low resolution structure of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by synchrotron small-angle X-ray scattering [J].
Hong, XG ;
Weng, YX ;
Li, M .
HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION, 2003, 27 :57-62
[44]   Protein Dynamics Tunes Excited State Positions in Light-Harvesting Complex II [J].
Vrandecic, Kamarniso ;
Raetsep, Margus ;
Wilk, Laura ;
Rusevich, Leonid ;
Golub, Maksym ;
Reppert, Mike ;
Irrgang, Klaus-Dieter ;
Kuehlbrandt, Werner ;
Pieper, Joerg .
JOURNAL OF PHYSICAL CHEMISTRY B, 2015, 119 (10) :3920-3930
[45]   Ultrafast excitation relaxation in light-harvesting complex LH2 from Rb. sphaeroides 601 [J].
Guo Lijun ;
Liu Yuan ;
Liu Weimin ;
Guo Junhua ;
Xu Chunhe ;
Qian Shixiong .
Science in China Series B: Chemistry, 2004, 47 :192-199
[46]   Ultrafast excitation relaxation in light-harvesting complex LH2 from Rb.sphaeroides 601 [J].
GUO Lijun LIU Yuan LIU Weimin GUO Junhua XU Chunhe QIAN Shixiong School of Physics and Information Optoelectronics Henan University Kaifeng China Physics Department Fudan University Shanghai China Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China .
Science in China(Series B), 2004, (03) :192-199
[47]   Ultrafast excitation relaxation in light-harvesting complex LH2 from Rb. sphaeroides 601 [J].
Guo, LJ ;
Liu, Y ;
Liu, WM ;
Guo, JH ;
Xu, CH ;
Qian, SX .
SCIENCE IN CHINA SERIES B-CHEMISTRY, 2004, 47 (03) :192-199
[48]   THE RELATIONSHIP BETWEEN CAROTENOID BIOSYNTHESIS AND THE ASSEMBLY OF THE LIGHT-HARVESTING LH2 COMPLEX IN RHODOBACTER-SPHAEROIDES [J].
LANG, HP ;
HUNTER, CN .
BIOCHEMICAL JOURNAL, 1994, 298 :197-205
[49]   Membrane protein stability: High pressure effects on the structure and chromophore-binding properties of the light-harvesting complex LH2 [J].
Gall, A ;
Ellervee, A ;
Sturgis, JN ;
Fraser, NJ ;
Cogdell, RJ ;
Freiberg, A ;
Robert, B .
BIOCHEMISTRY, 2003, 42 (44) :13019-13026
[50]   Static and Dynamic Disorder in Bacterial Light-Harvesting Complex LH2: A 2DES Simulation Study [J].
Rancova, Olga ;
Abramavicius, Darius .
JOURNAL OF PHYSICAL CHEMISTRY B, 2014, 118 (27) :7533-7540