Multi-photon microscopy in cardiovascular research

被引：18

作者：

Wu, Zhuojun ^{[1
,5
]}

Rademakers, Timo ^{[2
,3
]}

Kiessling, Fabian ^{[5
]}

Vogt, Michael ^{[6
]}

Westein, Erik ^{[7
]}

Weber, Christian ^{[4
,8
]}

Megens, Remco T. A. ^{[4
,8
]}

van Zandvoort, Marc ^{[1
,2
]}

机构：

[1] Rhein Westfal TH Aachen, Univ Clin, Inst Mol Cardiovasc Res, Pauwelsstr 30, D-52074 Aachen, Germany

[2] Maastricht Univ, Sch Cardiovasc Dis CARIM, Dept Mol Cell Biol, Univ Singel 50, NL-6229 ER Maastricht, Netherlands

[3] Maastricht Univ, Med Ctr, Dept Pathol, P Debyelaan 25, NL-6229 HX Maastricht, Netherlands

[4] Ludwig Maximillians Univ, Inst Cardiovasc Prevent, Pettenkoferstr 8a, D-80336 Munich, Germany

[5] Rhein Westfal TH Aachen, Univ Clin, Inst Expt Mol Imaging, Pauwelsstr 30, D-52074 Aachen, Germany

[6] Rhein Westfal TH Aachen, Univ Clin, Inst Lab Anim Sci & Expt Surg, Pauwelsstr 30, D-52074 Aachen, Germany

[7] Baker IDI Heart & Diabet Inst, Atherothrombosis & Vasc Biol, 75 Commercial Rd, Melbourne, Vic 3004, Australia

[8] Maastricht Univ, Sch Cardiovasc Dis CARIM, Univ Singel 50, NL-67229 ER Maastricht, Netherlands

来源：

METHODS | 2017年 / 130卷

关键词：

Optical imaging; Cardiovascular imaging; Carotid artery; Non-linear microscopy; Intravital imaging; JUNCTIONAL ADHESION MOLECULE; 2-PHOTON MICROSCOPY; ENDOTHELIAL GLYCOCALYX; ATHEROSCLEROTIC PLAQUES; HEART-FAILURE; BEATING HEART; LIVING CELLS; NITRIC-OXIDE; COLLAGEN; CONTRAST;

D O I：

10.1016/j.ymeth.2017.04.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiphoton laser scanning microscopy has proven profound value for ex vivo 3D histology and in vivo imaging of motionless tissue. The development of triggering systems and fast imaging methods, combined with advanced preparation procedures solved the challenging task of intravital imaging of the fast pulsating heart and major arteries in animals and further increased the popularity of intravital multiphoton imaging in cardiovascular research. This review article will highlight the potential of multiphoton microscopy for the visualization and characterization of dynamical and structural processes involved in cardiac and vascular diseases, both in an ex vivo and an intravital animal setting. Examples will be given how multiphoton microscopy can be applied to imaging of atherosclerotic plaque development and progression at subcellular level as well as to intravital imaging of inflammatory processes in the heart. In addition to highlighting the potential of multiphoton microscopy in preclinical cardiovascular research, we will discuss how this tool and its applications may be clinically translated to support disease diagnosis and therapy in patients. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：79 / 89

页数：11