The cancer/testis antigen CAGE-1 is a component of the acrosome of spermatids and spermatozoa

被引：18

作者：

Alsheimer, M ^{[1
]}

Drewes, T ^{[1
]}

Schütz, W ^{[1
]}

Benavente, R ^{[1
]}

机构：

[1] Univ Wurzburg, Bioctr, Dept Cell & Dev Biol, D-97074 Wurzburg, Germany

来源：

EUROPEAN JOURNAL OF CELL BIOLOGY | 2005年 / 84卷 / 2-3期

关键词：

cancer/testis antigens; spermatogenesis; acrosome; CAGE-1; SCP1;

D O I：

10.1016/j.ejcb.2004.11.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cancer/testis antigens (CTAs) are characterized by their restricted expression pattern. In normal individuals their expression is largely restricted to the testis. In the case of cancer patients, CTA expression has also been frequently observed in the tumoral cells. CTAs are considered to be promising targets for immunotherapy. However, almost nothing is known about the properties defined by the vast majority of CTAs. Here, we have investigated the expression pattern and localization of the CTA CAGE-1 during mouse spermatogenesis. We show that protein CAGE-1 is 849 amino acids long. Analysis of the first spermatogenic wave of pubertal mice by RT-PCR and immunoblotting showed that CAGE-I is predominantly expressed during postmeiotic stages. CAGE-1 localizes to the acrosomal matrix and acrosomal granule, as demonstrated by immunocytochemistry at the light and electron microscopic level. Taken together. Our results allowed to define protein CAGE-1 as a novel component of the acrosome of mammalian spermatids and spermatozoa. (c) 2004 Elsevier GmbH. All rights reserved.

引用

页码：445 / 452

页数：8

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