pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies

被引：7
|
作者：
Ng, Paul K. [1 ]
Snyder, Mark A. [1 ]
机构：
[1] Biorad Labs, Proc Applicat R&D, Proc Chromatog Div, Hercules, CA 94547 USA
来源：
JOURNAL OF SEPARATION SCIENCE | 2012年 / 35卷 / 01期
关键词：
Antibody; Cation exchange; Elution; Nuvia S; pH; SIMPLE BUFFER MIXTURES; PURIFICATION; GRADIENTS; CLEARANCE; COLUMNS;
D O I：
10.1002/jssc.201100720
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH-based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia (TM) S), which has high binding capacity. A three-column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50-cycle study.
引用
收藏
页码：29 / 35
页数：7
相关论文
共 50 条
  • [41] A method for suppressing pH excursion during elution in ion-exchange chromatography
    Ghosh, Raja
    JOURNAL OF CHROMATOGRAPHY OPEN, 2024, 6
  • [42] Development of an innovative salt-mediated pH gradient cation exchange chromatography method for the characterization of therapeutic antibodies
    Goyon, Alexandre
    McDonald, Daniel
    Fekete, Szabolcs
    Guillarme, Davy
    Stella, Cinzia
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2020, 1160
  • [43] Case study: Model-based chromatography characterization for a monoclonal antibody cation exchange step
    Hunt, Steve
    Larsen, Trent
    Tolley, Kevin
    Belval, Tom
    Todd, Robert
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 247
  • [44] Surface induced denaturation of monoclonal antibodies on cation exchange chromatographic media
    Gillespie, Ronald O.
    MacNeil, Sean
    Thao Nguyen
    Jones, Laurie
    Crampton, Shon
    Vunnum, Suresh
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 243
  • [45] The rapid identification of elution conditions for therapeutic antibodies from cation-exchange chromatography resins using high-throughput screening
    McDonald, Paul
    Tran, Benjamin
    Williams, Christopher R.
    Wong, Marc
    Zhao, Ti
    Kelley, Brian D.
    Lester, Philip
    JOURNAL OF CHROMATOGRAPHY A, 2016, 1433 : 66 - 74
  • [46] Comparative charge-based separation study with various capillary electrophoresis (CE) modes and cation exchange chromatography (CEX) for the analysis of monoclonal antibodies
    Kahle, Julia
    Zagst, Holger
    Wiesner, Rebecca
    Waetzig, Hermann
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2019, 174 : 460 - 470
  • [47] Weak partitioning chromatography for anion exchange purification of monoclonal antibodies
    Kelley, Brian D.
    Tobler, Scott A.
    Brown, Paul
    Coffman, Jonathan L.
    Godavarti, Ranga
    Iskra, Timothy
    Switzer, Mary
    Vunnum, Suresh
    BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (03) : 553 - 566
  • [48] Hydrophobic interaction chromatography at low salt concentration for the capture of monoclonal antibodies
    Kato, Y
    Nakamura, K
    Kitamura, T
    Hasegawa, M
    Sasaki, H
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1036 (01) : 45 - 50
  • [49] Cation-exchange chromatography of monoclonal antibodies Characterization of a novel stationary phase designed for production-scale purification
    Urmann, Marina
    Graalfs, Heiner
    Joehnck, Matthias
    Jacob, Lothar R.
    Frech, Christian
    MABS, 2010, 2 (04) : 395 - 404
  • [50] Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part II: pH gradient approach
    Fekete, Szabolcs
    Beck, Alain
    Fekete, Jeno
    Guillarme, Davy
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2015, 102 : 282 - 289
12345