pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies

被引：7

作者：

Ng, Paul K. ^{[1
]}

Snyder, Mark A. ^{[1
]}

机构：

[1] Biorad Labs, Proc Applicat R&D, Proc Chromatog Div, Hercules, CA 94547 USA

来源：

JOURNAL OF SEPARATION SCIENCE | 2012年 / 35卷 / 01期

关键词：

Antibody; Cation exchange; Elution; Nuvia S; pH; SIMPLE BUFFER MIXTURES; PURIFICATION; GRADIENTS; CLEARANCE; COLUMNS;

D O I：

10.1002/jssc.201100720

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH-based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia (TM) S), which has high binding capacity. A three-column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50-cycle study.

引用

页码：29 / 35

页数：7

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