Assessment of biochemical and immunomodulatory activity of sulphated polysaccharides from Ulva intestinalis

被引：58
作者：
Peasura, Napassorn [1 ]
Laohakunjit, Natta [1 ]
Kerdchoechuen, Orapin [1 ]
Vongsawasdi, Punchira [2 ]
Chao, Louis Kuoping [3 ]
机构：
[1] King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Div Biochem Technol, 49 Tientalay 25 Rd, Bangkok 10150, Thailand
[2] King Mongkuts Univ Technol Thonburi, Dept Microbiol, Fac Sci, 126 Pracha Uthit Rd, Bangkok 10140, Thailand
[3] China Med Univ, Dept Cosmeceut, 91 Hsueh Shih Rd, Taichung 40402, Taiwan
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 91卷
关键词：
Ulva intestinalis; Sulphated polysaccharide; Silica-silica column; Immunomodulatory activity; MOLECULAR-WEIGHT; IN-VITRO; ANTIOXIDANT ACTIVITIES; TUMOR-NECROSIS; POLYMANNUROGULURONATE; CYTOKINES; PLANTS; RED;
D O I：
10.1016/j.ijbiomac.2016.05.062
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The biochemical characteristics and immunomodulatory activity of sulphated polysaccharides isolated from Ulva intestinalis and fractionated using a silica-silica column were investigated. The unfractionated (USP) and fractionated sulphated polysaccharides (FSP4, FSP30, and FSP32) consisted mostly of carbohydrates (4.84-26.55%) and sulphates (2.85-20.42%). Structural analyses showed that USP, FSP4, FSP30 and FSP32 had molecular weights of 300, 80, 110 and 140 kDa, respectively. FSP30 exhibited the strongest DPPH radical scavenging activity. Moreover, FSP30 showed stronger immunomodulatory activities than UPS in term of stimulating the production of pro-inflammatory cytokines, including nitric oxide (NO), tumour necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta), in macrophage J774A.1 cells. USP and FSP30 were not cytotoxic to mouse macrophage at the tested concentrations (6.25-50 mu g/mL). The results suggested that U. intestinalis polysaccharides could be explored as potential antioxidant and immunomodulatory agents to be used as complementary medicine or functional foods. (C) 2016 Elsevier B.V. All rights reserved.
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收藏
页码：269 / 277
页数：9
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